Study M680
Study name | Zhang F 2020 |
Title | 5-Fluorouracil induced dysregulation of the microbiome-gut-brain axis manifesting as depressive like behaviors in rats |
Overall design | In this study, behavioral experiments were initially performed to determine whether the rats developed depression-like behaviors after 4 days 5-fluorouracil treatment. Sprague-Dawley rats were divided into the following 4 groups: (1) control group (vehicle injection, n = 10), (2) 5-fluorouracil group (5-fluorouracil intraperitoneal injection of 40 mg/kg once a day for 4 days, n = 10), (3) 5-fluorouracil + sham FMT group (5-fluorouracil intraperitoneal injection, and fed with PBS via oral gavage once a day for 4 days, n = 8), and (4) 5-fluorouracil + FMT group (5-fluorouracil intraperitoneal injection, and fed with the fecal suspension from normal rats via oral gavage once a day for 4 days, n = 8). Then, 1H nuclear magnetic resonance (NMR) spectroscopy-based metabolic phenotyping was used to investigate the changes of prefrontal cortex (PFC) metabolites affected by 5-fluorouracil. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Other animal model; Other animal model; |
Criteria for depression | Sucrose preference test, tail suspension test |
Sample size | 36 |
Tissue | Central; Brain; Prefrontal cortex; |
Platform | NMR; NMR: Bruker 600 MHz AVANCE III spectrometer; |
PMID | |
DOI | |
Citation | Zhang F, Chen H, Zhang R, et al. 5-Fluorouracil induced dysregulation of the microbiome-gut-brain axis manifesting as depressive like behaviors in rats. Biochim Biophys Acta Mol Basis Dis. 2020;1866(10):165884. |
Metabolite | myo-Inositol; Gamma-Aminobutyric acid; N-Acetyl-L-aspartic acid; L-Valine; L-Glutamic acid; Glycine; L-Isoleucine; L-Methionine; L-Alanine; L-Glutamine; L-Aspartic acid; Succinic acid; Choline; Fumaric acid; |
