Study M672
Study name | Xu T 2020 |
Title | Urinary metabolomics analysis of the anti-depressive effects of Hemerocallis citrina extracts in a simulated microgravity-induced rat model of depression |
Overall design | The aim of this study was to identify the metabolic products and metabolic pathways of Hemerocallis citrina extracts antidepressant and elucidate its mechanism, which might provide a theoretical foundation for the application of H. citrina in the treatment of depression. The depression model of rats was induced by 14-d simulated microgravity (SMG) treatment. Sprague-Dawley rats were divided into the following 4 groups (n = 8 in each group): (1) control group, (2) SMG group (simulated microgravity), (3) SMG + Hemerocallis citrina extracts group (simulated microgravity plus Hemerocallis citrina extracts treatment at the dose of 8 g/kg), and (4) SMG + paroxetine group (simulated microgravity plus paroxetine treatment at the dose of 10 mg/kg). The SMG stress procedure lasted for 14 days, and drugs were administered via intragastric after stress until the 39th day. Six urine samples per group were used for metabonomic analysis. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Other animal model; Other animal model; |
Criteria for depression | Not reported |
Sample size | 24 |
Tissue | Peripheral; Urine; Urine; |
Platform | MS-based; LC-MS: Waters ACQUITY Ultra Performance LC system with QTOF analyzer in a Xevo G2-XS system(Waters Corporation, Milford, MA, USA); Agilent 1290UHPLC-6495QQQ-MS/MS (Agilent Corporation, Palo Alto, CA, USA); |
PMID | |
DOI | |
Citation | Xu T, Wang Y, Lu C, et al. Urinary metabolomics analysis of the anti-depressive effects of Hemerocallis citrina extracts in a simulated microgravity-induced rat model of depression. J Chin Pharm Sci 2020; 29(3):176-191. |
Metabolite | Citric acid; L-Proline; L-Glutamic acid; L-Glutamine; Hippuric acid; 5-Hydroxyindoleacetic acid; Kynurenic acid; Phenylacetylglycine; Xanthurenic acid; Hydroxykynurenine; DOPA; Taurine; Creatine; Cyclic AMP; 3-Hydroxyhippuric acid; Indoleacetaldehyde; 2-Phenylethanol glucuronide; 5-Hydroxy-L-tryptophan; Adrenosterone; Deoxyuridine; 2-Isopropyl-3-oxosuccinate; |
