Study M608
Study name | Gao XX 2020 |
Title | Antidepressant-like effect of triterpenoids extracts from Poria cocos on the CUMS rats by 16S rRNA gene sequencing and LC-MS metabolomics |
Overall design | The aim of this study was to explore the antidepressant-like effect and a potential mechanism of the triterpenoids extracts from Poria cocos. 16S rRNA gene sequencing techniques coupled with the LC-MS metabolomics were performed to study the effects of Poria cocos on chronic unpredictable mild stress (CUMS) rats and the potential mechanism based on metabolomics and gut microbiota. Sprague-Dawley rats were divided into the following 3 groups (n = 6 in each group): (1) control group, (2) CUMS group, and (3) CUMS + Poria cocos group (stressor plus Poria cocos treatment at the dose of 15 g/kg). The CUMS stress procedure lasted for 4 weeks, and drugs were administered via intragastric once per day during the model building period. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test |
Sample size | 18 |
Tissue | Peripheral; Faece; Faece; |
Platform | MS-based; LC-MS: UHPLC-QExactive Orbitrap-MS (Thermo-Fisher Scientific, Waltham, MA, USA); |
PMID | |
DOI | |
Citation | Gao X, Feng Y, Xue H, et al. Antidepressant-like effect of triterpenoids extracts from Poria cocos on the CUMS rats by 16S rRNA gene sequencing and LC-MS metabolomics. J Liq Chromatogr Relat Technol. 2020. |
Metabolite | Citric acid; Oleic acid; Taurocholic acid; Cholic acid; Kynurenic acid; Taurine; Creatine; Deoxyadenosine; Stearoylcarnitine; Taurochenodesoxycholic acid; Octadecanamide; Deoxycholic acid; Benzoic acid; Spermidine; L-Palmitoylcarnitine; |
