Study M604
Study name | Feng Y 2020 |
Title | Multi-omics reveals the mechanisms of antidepressant-like effects of the low polarity fraction of Bupleuri Radix |
Overall design | In this study, LC-MS metabolomics combined with 16S rRNA gene sequencing technology analysis method to explore the mechanism of the low polarity fraction of Bupleuri Radix (PBR) in the treatment of depression from the perspective of multi-level integration of metabolites and gut microbiota. Chronic unpredictable mild stress (CUMS) model of depression was established. Sprague-Dawley rats were divided into the following 3 groups (n = 6 in each group): (1) control group, (2) CUMS group, and (3) CUMS + PBR group (stressor plus PBR treatment at the dose of 15 g/kg). The CUMS stress procedure lasted for 4 weeks, and drugs were administered via intragastric once per day during the model building period. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test |
Sample size | 18 |
Tissue | Peripheral; Faece; Faece; |
Platform | MS-based; LC-MS Waters ACQUITY UPLC with UHPLC-Q-Exactive Orbitrap-MS (Thermo-Fisher Scientific, USA); |
PMID | |
DOI | |
Citation | Feng Y, Gao X, Meng M, et al. Multi-omics reveals the mechanisms of antidepressant-like effects of the low polarity fraction of Bupleuri Radix. J Ethnopharmacol. 2020;256:112806. |
Metabolite | Citric acid; Oleic acid; Taurocholic acid; Cholic acid; Kynurenic acid; Taurine; Creatine; Deoxyadenosine; Stearoylcarnitine; Taurochenodesoxycholic acid; Octadecanamide; Deoxycholic acid; Benzoic acid; Spermidine; L-Palmitoylcarnitine; |
