Study M593

Study name

Chen CC 2020b

Title

[The mechanism of the anti-depression effect of the Radix Bupleuri-Radix Paeoniae Alba herb pair determined by liver metabolomics]

Overall design

The aim of this study was to analyzed the effect of the Radix Bupleuri-Radix Paeoniae Alba herb pair on endogenous metabolites in rats with chronic unpredictable mild stress (CUMS)-induced depression by using LC-MS liver metabolomics. Sprague-Dawley rats were divided into the following 5 groups (n = 10 in each group): (1) control group, (2) CUMS group, (3) CUMS + high dose of Radix Bupleuri-Radix Paeoniae Alba herb pair group (stressor plus Radix Bupleuri-Radix Paeoniae Alba herb pair treatment at the dose of 15 g/kg), (4) CUMS + low dose of Radix Bupleuri-Radix Paeoniae Alba herb pair group (stressor plus Radix Bupleuri-Radix Paeoniae Alba herb pair treatment at the dose of 7.5 g/kg), and (5) CUMS + venlafaxine group (stressor plus venlafaxine treatment at the dose of 35 mg/kg). The CUMS stress procedure lasted for 4 weeks, and drugs were administered via intragastric once per day during the model building period. Eight liver samples per group were used for LC-MS-based metabonomic analysis.

Study Type

Type1;

Type2;

Data available

Unavailable

Organism

Rat; Sprague-Dawley rat;

Categories of depression

Animal model; Chronic mild stress model; Chronic mild stress model;

Criteria for depression

Sucrose preference test, forced swimming test

Sample size

40

Tissue

Peripheral; Liver; Liver;

Platform

MS-based; LC-MS: Thermo-Fisher Dionex UltiMate 3000 UHPLC-Q Exactive Orbitrap-MS (Thermo Fisher, USA);

PMID

N/A

DOI

10.16438/j.0513-4870.2019-0731

Citation

Chen C, Yin Q, Tian J, et al. [The mechanism of the anti-depression effect of the Radix Bupleuri-Radix Paeoniae Alba herb pair determined by liver metabolomics]. Acta Pharmaceutica Sinica 2020;55(5):941-949. (Article in Chinese)

Metabolite

Citric acid;

L-Tryptophan;

L-Glutamic acid;

Arachidonic acid;

L-Lysine;

L-Tyrosine;

L-Arginine;

Pantothenic acid;

Oxoglutaric acid;

Ascorbic acid;

Fumaric acid;

Uracil;

Creatine;

Niacinamide;

Ornithine;

Carbamoyl phosphate;

Glycocholic acid;

Glutathione;

Glycoursodeoxycholic acid;

Taurochenodesoxycholic acid;

L-Norleucine;

4-(3-hydroxyprop-1-en-1-yl)phenol;

4-Oxoproline;

3'-AMP;

2-Hexenoylcarnitine;