Study M581

Study name

Zhang YW 2019

Title

An integrated strategy for ascertaining quality marker of Schisandra chinensis (Turcz.) Baill based on correlation analysis between depression-related monoaminergic metabolites and chemical components profiling

Overall design

In this study, rats were exposed to the chronic unpredictable mild stress (CUMS) to induce depression, then the monoaminergic metabolites contained in noradrenergic, dopaminergic and serotonergic pathways were selected to screen potential biomarkers of depression treated by Schisandra chinensis (Turcz.) Baill (S. chinensis) based on target metabolomics using a rapid HPLC-MS/MS method. Sprague-Dawley rats were divided into the following 4 groups (n = 6 in each group): (1) control group, (2) CUMS group, (3) CUMS + high dose of S. chinensis group (stressor plus Schisandra chinensis Baill treatment at the dose of 900 mg/kg), and (4) CUMS + fluoxetine group (stressor plus fluoxetine treatment at the dose of 10 mg/kg). The CUMS stress procedure lasted for 4 weeks, and drugs were administered via intragastric once per day during the model building period.

Study Type

Type1;

Type2;

Data available

Unavailable

Organism

Rat; Sprague-Dawley rat;

Categories of depression

Animal model; Chronic mild stress model; Chronic mild stress model;

Criteria for depression

Forced swimming test

Sample size

24

Tissue

Central; Brain; Hippocampus;

Central; Brain; Hypothalamus;

Central; Brain; Prefrontal cortex;

Platform

MS-based; LC-MS: QTRAPTM 4000 MS/MS system (AB Sciex, USA) with XR LC-20AD ProminenceTM HPLC system (Shimadzu, Japan);

PMID

31047662

DOI

10.1016/j.chroma.2019.03.056

Citation

Zhang Y, Lv X, Liu R, et al. An integrated strategy for ascertaining quality marker of Schisandra chinensis (Turcz.) Baill based on correlation analysis between depression-related monoaminergic metabolites and chemical components profiling. J Chromatogr A. 2019;1598:122-131.

Metabolite

Gamma-Aminobutyric acid;

L-Tryptophan;

L-Glutamic acid;

Homovanillic acid;

L-Tyrosine;

3,4-Dihydroxybenzeneacetic acid;

Serotonin;

DOPA;

Norepinephrine;

Dopamine;

Epinephrine;

3-Methoxytyramine;

Acetylcholine;

Methoxyepinephrine;