Study M523
Study name | Xu HR 2018 |
Title | Targeted neurotransmitters profiling identifies metabolic signatures in rat brain by LC-MS/MS: application in insomnia, depression and Alzheimer's Disease |
Overall design | In this study, a rapid, sensitive and reliable LC-MS/MS method was established for simultaneous determination of the NTs and their metabolites, including tryptophan (Trp), tyrosine (Tyr), serotonin (5-HT), 5-hydroxyindolacetic acid (5-HIAA), dopamine (DA), acetylcholine (ACh), norepinephrine (NE), glutamic acid (Glu), and gamma-aminobutyric acid (GABA) in rat brain tissues. Rats were divided into the following 2 groups (n = 8 in each group): (1) control group, (2) CUMS group. The CUMS stress procedure lasted for 3 weeks. |
Study Type | Type1; |
Data available | Unavailable |
Organism | Rat; Wistar rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Not reported |
Sample size | 16 |
Tissue | Central; Brain; Brain; |
Platform | MS-based; LC-MS: LC-20 A Prominence™ UFLC XR system and 4000 QTRAP triple quadrupole-linear ion trap mass spectrometry system; |
PMID | |
DOI | |
Citation | Xu H, Wang Z, Zhu L, et al. Targeted neurotransmitters profiling identifies metabolic signatures in rat brain by LC-MS/MS: application in insomnia, depression and Alzheimer's Disease. Molecules. 2018 Sep 17;23(9). pii: E2375. |
Metabolite |
