Study M516
Study name | Xu T 2019 |
Title | Liquid chromatography-mass spectrometry-based urinary metabolomics study on a rat model of simulated microgravity-induced depression |
Overall design | The aim of this study was to examine the metabolic profile of urine samples from a rat model of depression for identifying potential metabolic biomarkers associated with depression. The depression model of rats was induced by 14-d simulated microgravity (SMG) treatment. Principal component analysis and orthogonal partial least squares discriminate analysis were performed to classify and identify the differences of endogenous metabolites in urine between the normal and depressed rats. Rats were randomly divided into the following 2 groups (n = 8 in each group): (1) control group, (2) SMG-treated group. Control rats were kept together, whereas SMG-treated rats were housed separately by tail suspension using a specific tail suspension device throughout the experiment. |
Study Type | Type1; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Other animal model; Other animal model; |
Criteria for depression | Not reported |
Sample size | 16 |
Tissue | Peripheral; Urine; Urine; |
Platform | MS-based; LC-MS: Waters ACQUITY Ultra Performance LC system with Xevo G2-XS system (Waters Corporation, Milford, MA, USA); Agilent 1290UHPLC-6495QQQ-MS/MS (Agilent Corporation, CA, USA); |
PMID | |
DOI | |
Citation | Xu T, Lu C, Feng L, et al. Liquid chromatography-mass spectrometry-based urinary metabolomics study on a rat model of simulated microgravity-induced depression. J Pharm Biomed Anal. 2019 Feb 20;165:31-40. |
Metabolite | Citric acid; L-Proline; L-Glutamic acid; L-Glutamine; Hippuric acid; 5-Hydroxyindoleacetic acid; Kynurenic acid; Xanthurenic acid; |
