Study M492
Study name | Zhao L 2018 |
Title | A urinary metabolomics (GC-MS) strategy to evaluate the antidepressant-like effect of chlorogenic acid in adrenocorticotropic hormonetreated rats |
Overall design | The aim of this study was to investigate the antidepressant-like effects of chlorogenic acid (CGA) on an adrenocorticotropic hormone (ACTH)-treated rat model. Rats were divided into the following 4 groups (n = 8 in each group): (1) control group, (2) ACTH group, (3) ACTH + memantine group (ACTH plus memantine treatment at the dose of 30 mg/kg i.g.), and (4) ACTH + CGA group (ACTH plus CGA treatment at the dose of 500 mg/kg i.g.). Each rat in these three groups received s.c. injection of ACTH at a dose of 100 mg per rat for 14 days. Sucrose preference tests (SPTs) and open-field tests (OFTs) were performed to evaluate depressive-like behaviors. Non-target urinary metabolomic strategy based on GC-MS detection was applied on the profiling of global metabolomic character of ACTH-treated rats as well as ACTH-treated rats administrated with memantine and CGA. Six samples per group were used for metabonomic analysis. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Wistar rat; |
Categories of depression | Animal model; Other animal model; Other animal model; |
Criteria for depression | Sucrose preference test |
Sample size | 24 |
Tissue | Peripheral; Urine; Urine; |
Platform | MS-based; GC-MS: GC-TOFMS (Pegasus HT, Leco Corp., St. Joseph, MI); |
PMID | |
DOI | |
Citation | Zhao L, Zhang Z, Zhou M, et al. A urinary metabolomics (GC-MS) strategy to evaluate the antidepressant-like effect of chlorogenic acid in adrenocorticotropic hormonetreated rats. RSC Adv. 2018, 8, 9141. |
Metabolite | myo-Inositol; Gamma-Aminobutyric acid; L-Proline; L-Serine; L-Threonine; L-Alanine; L-Asparagine; Hippuric acid; Oxalic acid; Urea; Kynurenic acid; |
