Study M473
Study name | Hassan AM 2019 |
Title | High-fat diet induces depression-like behaviour in mice associated with changes in microbiome, neuropeptide Y, and brain metabolome |
Overall design | The aim of this study was to examine the impact of high-fat diet (HFD) on murine behaviour, intestinal microbiome, brain metabolome, neuropeptide Y (NPY) expression, and dipeptidyl peptidase-4 (DPP-4) activity. Male C57Bl/6J mice were fed an HFD (60 kJ% from fat, high-fat diet group) or control diet (12 kJ% from fat, control group) for 8 weeks, followed by behavioural phenotyping. Caecal microbiome was analysed by 16S rDNA sequencing, brain metabolome by 1H nuclear magnetic resonance, NPY expression by PCR and immunoassay, and dipeptidyl peptidase-4 (DPP-4) activity by enzymatic assay. |
Study Type | Type1; |
Data available | Unavailable |
Organism | Mouse; C57BL/6J mouse; |
Categories of depression | Animal model; Other animal model; Other animal model; |
Criteria for depression | Social interaction test, sucrose preference test |
Sample size | 24 |
Tissue | Central; Brain; Prefrontal cortex; Central; Brain; Hippocampus; Central; Brain; Striatum; Central; Brain; Hypothalamus; |
Platform | NMR; NMR: Bruker AV700 NMR Spectrometer; |
PMID | |
DOI | |
Citation | Hassan AM, Mancano G, Kashofer K, et al. High-fat diet induces depression-like behaviour in mice associated with changes in microbiome, neuropeptide Y, and brain metabolome. Nutr Neurosci. 2019 Dec;22(12):877-893. |
Metabolite | myo-Inositol; Gamma-Aminobutyric acid; N-Acetyl-L-aspartic acid; |
