Study M472
Study name | Faccio AT 2018 |
Title | Stereochemical and structural effects of (2R,6R)-hydroxynorketamine on the mitochondrial metabolome in PC-12 cells |
Overall design | In this study a multi-platform, non-targeted metabolomics approach was used to study the change in mitochondrial metabolome of PC-12 cells treated with ketamine and (2S,6S;2R,6R)-hydroxynorketamine (HNK) enantiomers. The identified metabolites were grouped into pathways in order to assess global responses. The spent Medium was replaced with fresh Medium (control group) or with Medium containing 1000 nM (R)-ketamine (R-Ket group), 1000 nM (S)-ketamine (S-Ket group), 5 nM (2R,6R)-HNK ((2R,6R)-HNK group) or 0.5 nM (2S,6S)-HNK ((2S,6S)-HNK group). |
Study Type | Type3; |
Data available | Unavailable |
Organism | Rat; Rat; |
Categories of depression | Healthy individuals; Healthy individuals; Healthy individuals; |
Criteria for depression | Not reported |
Sample size | N/A |
Tissue | Central; Brain; PC12 cell; |
Platform | MS-based; CE-MS: capillary electrophoresis system (7100 CE, Agilent Technologies) with time-of-flight mass spectrometer (6224 TOF/MS system, Agilent Technologies); MS-based; GC-MS: gas chromatograph (Agilent Technologies 7890A GC) with quadrupole mass spectrometer (Agilent Technologies 5975 inert MSD); |
PMID | |
DOI | |
Citation | Faccio AT, Ruperez FJ, Singh NS, et al. Stereochemical and structural effects of (2R,6R)-hydroxynorketamine on the mitochondrial metabolome in PC-12 cells. Biochim Biophys Acta Gen Subj. 2018;1862(6):1505-15. |
Metabolite | Glyceric acid; myo-Inositol; Citric acid; Linoleic acid; Glycerol; Oleic acid; Stearic acid; Palmitoleic acid; Palmitic acid; L-Leucine; L-Proline; L-Glutamic acid; Glycine; L-Serine; L-Threonine; Arachidonic acid; L-Lactic acid; L-Lysine; L-Arginine; Uridine; Dimethylglycine; Oxoglutaric acid; Fructose 6-phosphate; Glucose 6-phosphate; Uracil; Docosahexaenoic acid; D-Ribose; 7-Methylxanthine; Citrulline; Elaidic acid; Inosinic acid; L-Carnitine; Adenine; Cytidine; Guanosine; Spermidine; Glycerol 1-hexadecanoate; |
