Study M209
Study name | Zhang YQ 2017 |
Title | Integrated metabolomics and proteomics analysis of hippocampus in a rat model of depression |
Overall design | In this study, we applied an integrated analysis of gas chromatography/mass spectrometry (GC-MS)-based metabolomics and isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomics to investigate changes in the hippocampus in the chronic unpredictable mild stress (CUMS) rat model of depression. Only the stress-susceptible rats in the CUMS group were selected for profiling against controls. Rats were divided into 2 groups: (1) control group, (2) CUMS group. Nine sample in the CUMS group and eight sample in the control group were used for metabonomic analysis. The CUMS stress procedure lasted for 3 weeks. |
Study Type | Type1; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Forced swimming test |
Sample size | 17 |
Tissue | Central; Brain; Hippocampus; |
Platform | MS-based; GC-MS: Agilent 7890A/5975C GC/MSD System (Agilent Technologies, CA, USA); |
PMID | |
DOI | |
Citation | Zhang Y, Yuan S, Pu J, et al. Integrated metabolomics and proteomics analysis of hippocampus in a rat model of depression. Neuroscience 2017;371:207-20. |
Metabolite | myo-Inositol; Glycerol; Pyroglutamic acid; N-Acetyl-L-aspartic acid; L-Cysteine; L-Glutamic acid; Arachidonic acid; L-Lactic acid; L-Asparagine; L-Glutamine; Succinic acid; Ascorbic acid; Ethanolamine; Taurine; Creatine; Cholesterol; 3-Phosphoglyceric acid; Oxamate; Phosphate; O-Phosphoethanolamine; L-Allothreonine; Lipoamide; Fosfomycin; Methyl palmitoleate; N-Methylhydantoin; MG(0:0/16:0/0:0); |
