Study M204
Study name | Wu Y 2017 |
Title | Imbalance in amino acid and purine metabolisms at the hypothalamus in inflammation-associated depression by GC-MS |
Overall design | In this study, taking advantage of the well-established lipopolysaccharide (LPS)-induced depression mouse model, metabolic changes were measured in the hypothalamus using gas chromatography-mass spectrometry (GC-MS). Mice were divided into the following 2 groups (n = 10 in each group): (1) control group, (2) LPS group (0.83mg/kg lipopolysaccharide i.p.). Depressive-like behaviors were assessed at 24 h post-LPS i.p. administration. As soon as the behavioral tests were finished, the mice were sacrificed and the whole brain was removed. |
Study Type | Type1; |
Data available | http://www.rsc.org/suppdata/c7/mb/c7mb00494j/c7mb00494j1.zip |
Organism | Mouse; CD-1 mouse; |
Categories of depression | Animal model; Lipopolysaccharide induced depression model; Lipopolysaccharide induced depression model; |
Criteria for depression | Sucrose preference test, forced swimming test, tail suspension test |
Sample size | 20 |
Tissue | Central; Brain; Hypothalamus; |
Platform | MS-based; GC-MS: Agilent 7890A/5975C GC/MSD system with Agilent 5975C mass selective detector (Agilent Technologies, Santa Clara, CA, USA); |
PMID | |
DOI | |
Citation | Wu Y, Li Y, Jia Y, et al. Imbalance in amino acid and purine metabolisms at the hypothalamus in inflammation-associated depression by GC-MS. Mol Biosyst 2017;13(12):2715-28. |
Metabolite | myo-Inositol; Gamma-Aminobutyric acid; L-Cysteine; L-Tryptophan; L-Proline; L-Threonine; L-Alanine; L-Glutamine; L-Aspartic acid; Succinic acid; Urea; Phytosphingosine; Hydroxylamine; Hypoxanthine; Vanillylmandelic acid; DOPA; L-Histidine; Niacinamide; Inosine; Adenosine monophosphate; 3-Aminoisobutanoic acid; |
