Study M136
Study name | Xing H 2015 |
Title | Determination of depression biomarkers in rat plasma by liquid chromatography-mass spectrometry for the study of the antidepressant effect of Zhi-Zi-Hou-Po decoction on rat model of chronic unpredictable mild stress |
Overall design | The present study was designed to develop a liquid chromatography-mass spectrometry (LC-MS) method for simultaneous determination of depression biomarkers: tryptophan (Trp), phenylalanine (Phe), tyrosine (Tyr), indole-3-acetic acid (IAA), hippuric acid (HA), phenaceturic acid (PA), creatinine (Cr), glutamic acid (Glu), succinic acid (SA) and gama-aminobutyric acid (GABA), as well as to study the antidepressant effect and potential mechanism of Zhi-Zi-Hou-Po decoction (ZZHPD) based on holistic view of an organism. All the rats were divided into 5 groups (n = 8 in each group): (1) control group, (2) CUMS group, (3) CUMS + fluoxetine group (stress plus fluoxetine treatment at the dose of 15 mg/kg), (4) CUMS + high dose of ZZHPD group (stress plus Zhi-Zi-Hou-Po decoction treatment at the dose of 14.64 g/kg), and (5) CUMS + middle dose of ZZHPD group (stress plus Zhi-Zi-Hou-Po decoction treatment at the dose of 7.32 g/kg). The CUMS stress procedure lasted for 5 weeks, and drugs were administered by gastric gavages once daily during the last 3 weeks of the model building period. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test |
Sample size | 40 |
Tissue | Peripheral; Blood; Plasma; |
Platform | MS-based; LC-MS: LC-MS 2010EV system (Shimadzu, Japan); |
PMID | |
DOI | |
Citation | Xing H, Zhang K, Zhang R, et al. Determination of depression biomarkers in rat plasma by liquid chromatography-mass spectrometry for the study of the antidepressant effect of Zhi-Zi-Hou-Po decoction on rat model of chronic unpredictable mild stress. J Chromatogr B Analyt Technol Biomed Life Sci 2015; 988:135-42. |
Metabolite | Gamma-Aminobutyric acid; L-Phenylalanine; L-Tryptophan; |
