Study M126
Study name | Walker AK 2013 |
Title | NMDA receptor blockade by ketamine abrogates lipopolysaccharide-induced depressive-like behavior in C57BL/6J mice |
Overall design | Using mass-spectrometry (LC-MS) analysis of kynurenine metabolites in the brain of mice injected at the periphery with 1 mg/kg lipopolysaccharide (LPS), we show that LPS activates the kynurenine 3-monooxygenase pathway that ultimately degrades kynurenine into quinolinic acid. Mice were divided into 2 groups: (1) control group (single dose of sterile endotoxin-free isotonic PBS intraperitoneally, n = 10), (2) LPS group (single dose of 1 mg/kg LPS intraperitoneally, n = 8). Brains were collected 24 h thereafter. Liquid chromatography coupled with LC-MS was used to determine brain levels of tryptophan, kynurenine metabolites, serotonin, and 5-hydroxyindoleacetic acid (5-HA). |
Study Type | Type1; |
Data available | Unavailable |
Organism | Mouse; CD-1 mouse; |
Categories of depression | Animal model; Lipopolysaccharide induced depression model; Lipopolysaccharide induced depression model; |
Criteria for depression | Not repored |
Sample size | 18 |
Tissue | Central; Brain; Brain; |
Platform | MS-based; LC-MS: Waters Acquity HPLC system with Waters Quattro Premier XE triple quadrupole mass spectrometer; |
PMID | |
DOI | |
Citation | Walker AK, Budac DP, Bisulco S, et al. NMDA receptor blockade by ketamine abrogates lipopolysaccharide-induced depressive-like behavior in C57BL/6J mice. Neuropsychopharmacology 2013;38(9):1609-16. |
Metabolite | L-Tryptophan; 5-Hydroxyindoleacetic acid; |
