Study M120
Study name | Zheng SN 2011 |
Title | An 1H NMR and UPLC-MS-based plasma metabonomic study to investigate the biochemical changes in chronic unpredictable mild stress model of depression |
Overall design | In this study Sprague-Dawley rats were exposed to chronic unpredictable mild stress (CUMS) to induce depression. A metabonomic study on plasma of CUMS-induced depressive rats was performed to research the pathologic mechanism of depression by using 1H nuclear magnetic resonance (NMR) spectroscopy and ultra performance liquid chromatography coupled to mass spectrometry (UPLC-MS). Rats were divided into 2 groups (n = 8 in each group): (1) control group, (2) CUMS group. The CUMS stress procedure lasted for 11 weeks. |
Study Type | Type1; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test |
Sample size | 16 |
Tissue | Peripheral; Blood; Plasma; |
Platform | NMR; NMR: Bruker AV 600 spectrometer (Bruker Biospin, Germany); MS-based; LC-MS: Waters ACQUITY ultra performance liquid chromatography (UPLC) system with Micromass Quattro Micro API mass spectrometer (Waters Corp., Milford, MA, USA); |
PMID | |
DOI | |
Citation | Zheng S, Zhang S, Yu M, et al. An 1H NMR and UPLC-MS-based plasma metabonomic study to investigate the biochemical changes in chronic unpredictable mild stress model of depression. Metabolomics 2011;7:413-23. |
Metabolite | 3-Hydroxybutyric acid; L-Phenylalanine; L-Tryptophan; L-Valine; L-Lactic acid; L-Glutamine; Indole-3-propionic acid; D-Glucose; Choline; Cholic acid; LysoPC(16:0); |
