Study M114
Study name | Sun L 2017 |
Title | Biochemical effects of venlafaxine on astrocytes as revealed by 1H NMR-based metabolic profiling |
Overall design | Here, we used a 1H nuclear magnetic resonance (NMR)-based metabonomics method coupled with multivariate statistical analysis to characterize the metabolic profiling of astrocytes treated with venlafaxine to explore the potential mechanism of its antidepressant effect. For 1H NMR measurements, cells were cultured in 60 separate 10 cm dishes at a density of 2 x 106 cells per dish coated in 100 mm dishes, 30 of which were treated with venlafaxine (venlafaxine group, the astrocytes were exposed to venlafaxine solution 1-10 uM for 72 h), and 30 with dimethylsulphoxide (DMSO) as a vehicle control (control group). |
Study Type | Type3; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Healthy individuals; Healthy individuals; Healthy individuals; |
Criteria for depression | Not reported |
Sample size | 60 |
Tissue | Central; Brain; Astrocyte; |
Platform | NMR; NMR: Bruker AVANCE III 600 spectrometer; |
PMID | |
DOI | |
Citation | Sun L, Fang L, Lian B, et al. Biochemical effects of venlafaxine on astrocytes as revealed by 1H NMR-based metabolic profiling. Mol Biosyst 2017;13(2):338-49. |
Metabolite | myo-Inositol; L-Glutamic acid; L-Methionine; L-Lactic acid; L-Asparagine; L-Glutamine; L-Lysine; L-Tyrosine; Sebacic acid; Cholic acid; Fructose 6-phosphate; Ethanolamine; Betaine; Creatine; Beta-D-Glucose; VLDL; Unsaturated lipid; alpha-D-Glucose; Lipid; 3-Phosphoglyceric acid; Phosphorylcholine; Acetone; Isobutyric acid; Glycerol 3-phosphate; |
