Study M1098
Study name | Zhu YR 2023 |
Title | Untargeted and targeted mass spectrometry reveal the effects of theanine on the central and peripheral metabolomics of chronic unpredictable mild stress-induced depression in juvenile rats |
Overall design | The aim of this study was to explore the antidepressive effect of L-theanine and its related mechanisms in the chronic unpredictable mild stress (CUMS) model of depression. Sprague-Dawley rats were divided into the following 5 groups (n = 6 in each group): (1) control group, (2) CUMS group, (3) L-theanine group (stress with L-theanine treatment at the dose of 10 mg/kg), (4) CUMS + fluoxetine group (stress with fluoxetine treatment at the dose of 2 mg/kg), and (5) CUMS + L-theanine group (stress with L-theanine treatment at the dose of 10 mg/kg). Untargeted reversed-phase liquid chromatography-quadrupole-time-of-flight mass spectrometry and targeted hydrophilic interaction liquid chromatography-triple quadrupole mass spectrometry were developed to analyze the metabolism changes in the serum and hippocampus to screen for potential biomarkers related to L-theanine treatment. |
Study Type | Type1; Type2; Type3; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; Healthy individuals; Healthy individuals; Healthy individuals; |
Criteria for depression | Sucrose preference test, forced swimming test |
Sample size | 30 |
Tissue | Peripheral; Blood; Serum; Central; Brain; Hippocampus; |
Platform | MS-based; LC-MS: ACQUITY UPLCI-Class UPLC system with high-resolution Xevo G2 XS Q-Tof-MS/MS (Waters, Milford, MA, USA); MS-based; LC-MS: Agilent 1260 HPLC system (Agilent, Palo Alto, CA, USA) with SCIEX Triple Quad 4500 liquid chromatography-tandem mass spectrometry system (SCIEX, Framingham, MA, USA); |
PMID | |
DOI | |
Citation | Zhu Y, Wang F, Han J, et al. Untargeted and targeted mass spectrometry reveal the effects of theanine on the central and peripheral metabolomics of chronic unpredictable mild stress-induced depression in juvenile rats. J Pharm Anal. 2023 Jan;13(1):73-87. |
Metabolite | L-Phenylalanine; L-Tryptophan; L-Glutamic acid; L-Glutamine; L-Tyrosine; Uridine; Threonic acid; Phenylacetylglycine; Serotonin; Hypoxanthine; Vanylglycol; Xanthine; Creatine; Norepinephrine; Dopamine; Glycerophosphocholine; Cytidine; Corticosterone; Sphingosine; PS(18:0/22:6(4Z,7Z,10Z,13Z,16Z,19Z)); 5-Hydroxy-L-tryptophan; PE(18:3(6Z,9Z,12Z)/22:6(4Z,7Z,10Z,13Z,16Z,19Z)); PI(18:0/20:4(5Z,8Z,11Z,14Z)); LysoPC(P-18:1(9Z)); LysoPC(22:1(13Z)/0:0); Enkephalin L; PC(24:1(15Z)/24:1(15Z)); 5-Methyltetrahydrofolic acid; PC(15:0/18:1(11Z)); |
