Study M1096

Study name

Zhang ZW 2023

Title

Gut microbiota-based metabolites of Xiaoyao Pills (a typical Traditional Chinese medicine) ameliorate depression by inhibiting fatty acid amide hydrolase levels in brain

Overall design

The aim of this study was to explore the underlying antidepressant mechanism of Xiaoyao Pills in the chronic unpredictable mild stress (CUMS) model of depression. Sprague-Dawley rats were divided into the following 6 groups (n = 10 in each group): (1) control group, (2) CUMS group, (3) CUMS + fluoxetine group (stressor plus fluoxetine treatment at the dose of 10 mg/kg), (4) CUMS + low dose of Xiaoyao Pills group (stressor plus Xiaoyao Pills treatment at the dose of 0.75 g/kg), (5) CUMS + middle dose of Xiaoyao Pills group (stressor plus Xiaoyao Pills treatment at the dose of 1.5 g/kg), and (6) CUMS + high dose of Xiaoyao Pills group (stressor plus Xiaoyao Pills treatment at the dose of 3 g/kg). The CUMS stress procedure lasted for 7 weeks, and drugs were administered via intragastric once per day for 4 weeks after stress. Brain samples were used for untargeted and targeted metabolomics analysis (n=6-9/group).

Study Type

Type1;

Type2;

Data available

Unavailable

Organism

Rat; Sprague-Dawley rat;

Categories of depression

Animal model; Chronic mild stress model; Chronic mild stress model;

Criteria for depression

Sucrose preference test, forced swimming test

Sample size

39

Tissue

Central; Brain; Brain;

Platform

MS-based; GC-MS: Shimadzu GC-2010 Plus gas chromatography system with GCMS-QP2020 SE single quadruple mass spectrometer (Shimadzu, Kyoto, Japan);

MS-based; LC-MS: HPLC-MS/MS 8060 (Shimadzu Corporation, Japan);

PMID

37100263

DOI

10.1016/j.jep.2023.116555

Citation

Zhang ZW, Han P, Fu J, et al. Gut microbiota-based metabolites of Xiaoyao Pills (a typical Traditional Chinese medicine) ameliorate depression by inhibiting fatty acid amide hydrolase levels in brain. J Ethnopharmacol. 2023 Sep 15;313:116555.

Metabolite

Gamma-Aminobutyric acid;

Oleic acid;

Stearic acid;

Palmitic acid;

L-Proline;

L-Serine;

Arachidonic acid;

L-Aspartic acid;

L-Lysine;

Urea;

Hypoxanthine;

Fumaric acid;

Uracil;

Norepinephrine;

Dopamine;

Oleamide;

Anandamide;