Study M1042
Study name | Jiang WX 2023 |
Title | Microbiota-derived short-chain fatty acids may participate in post-stroke depression by regulating host's lipid metabolism |
Overall design | The aim of this study was to explore how disordered gut microbes participate in the pathogenesis of post-stroke depression through the microbiota-gut-brain axis. In this study, transient middle cerebral artery occlusion (MCAO) combined with chronic unpredictable mild stress (CUMS) was used as the post-stroke depression model. Sprague-Dawley rats were divided into the following 3 groups: (1) control group (sham MCAO surgery without stress), (2) MCAO group (MCAO surgery without stress), and (3) CUMS + MCAO group (MCAO surgery with stress). The CUMS stress procedure lasted for 4 weeks, after MCAO surgery. Fecal metabolome analysis was performed to identify the functional metabolites in the gut microbiome of rats. Then, the lipid metabolic signatures in the prefrontal cortex were conducted by liquid chromatography mass spectrometry. Six samples per group were used for metabonomic analysis. |
Study Type | Type1; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Other animal model; Other animal model; |
Criteria for depression | Sucrose preference test, forced swimming test |
Sample size | 18 |
Tissue | Central; Brain; Prefrontal cortex; Peripheral; Faece; Faece; |
Platform | MS-based; LC-MS: Q Exactive hybrid quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific Inc); MS-based; GC-MS: Agilent 7890A/5975C; |
PMID | |
DOI | |
Citation | Jiang W, Chen J, Gong L, et al. Microbiota-derived short-chain fatty acids may participate in post-stroke depression by regulating host's lipid metabolism. J Psychiatr Res. 2023 May;161:426-434. |
Metabolite | Oleic acid; L-Valine; L-Glutamic acid; L-Isoleucine; L-Tyrosine; Gamma-Tocopherol; Acetic acid; Aminoadipic acid; PC(37:4); Butyric acid; Valeric acid; PE(38:4); PS(38:4); PC(40:7); PC(18:1/22:6); PC(16:0/18:1); PS(41:1); PG(18:0/18:1); 2-Hydroxyvaleric acid; Hexacosanoic acid; Lactobionic acid; Stigmasterol; PC(18:0/22:6); Squalene; DG(36:3p); TG(16:0/16:0/21:5); TG(16:0/22:6/22:6); TG(18:0/22:6/22:6); TG(20:3/22:6/22:6); PC(33:0); TG(16:0/16:1/22:6); Cer(d38:0); SM(d34:4); CerG1(d44:4); Docosenoic acid; DG(54:6); DG(34:2p); DG(34:1/18:1); DG(16:0/22:6); TG(18:0/16:0/20:4); TG(58:6e); TG(56:7e); TG(26:1/18:1/18:2); TG(24:5/18:0/18:0); TG(20:1/18:1/18:1); TG(20:0/20:4/20:4); TG(18:1p/18:0/24:7); TG(18:1/22:6/22:6); TG(18:0e/16:0/22:6); TG(18:0/18:1/22:5); TG(18:0/16:0/22:4); TG(18:0/16:0/18:1); TG(16:0e/16:0/22:6); TG(16:0/22:4/22:6); TG(16:0/20:4/22:6); TG(16:0/20:1/20:3); TG(16:0/16:0/22:6); PMe(49:1); dMePE(38:6p); PG(16:0/22:4); PS(46:13); PS(46:12); PS(39:1); PE(38:7); PE(20:1/20:4); PC(44:7); PC(38:4e); PC(22:6/22:6); |
