Study M1004
Study name | Liu JL 2022a |
Title | Butyrate emerges as a crucial effector of Zhi-Zi-Chi decoctions to ameliorate depression via multiple pathways of brain-gut axis |
Overall design | The aim of this study was to explore the antidepressant mechanism of Zhi-Zi-Chi decoctions underlying brain-gut axis in the chronic unpredictable mild stress (CUMS) model of depression. Sprague-Dawley rats were divided into the following 4 groups: (1) control group, (2) CUMS group, (3) CUMS + fluoxetine group (stressor plus fluoxetine treatment at the dose of 2.1 mg/kg), and (4) CUMS + Zhi-Zi-Chi decoctions group (stressor plus Zhi-Zi-Chi decoctions treatment at the dose of 10 g/kg). The CUMS stress procedure lasted for 6 weeks, and drugs were administered via intragastric during the last 4 weeks of the model building period. Cecal contents of short-chain fatty acids were measured by LC-MS (n = 6/group). The levels of neurotransmitters and amino acids in plasma, ileum, and hippocampus were also detected by LC-MS (n = 6/group). |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test, forced swimming test, tail suspension test |
Sample size | 24 |
Tissue | Peripheral; Gut; Ileum; Central; Brain; Hippocampus; Peripheral; Blood; Plasma; Peripheral; Faece; Faece; |
Platform | MS-based; LC-MS: Shimadzu LC-40D XS system with Shimadzu 8045 Triple Quadrupole Mass Spectrometer; MS-based; LC-MS: Agilent 1290 Infinity LC system with Agilent 6538 Accurate Mass Quadrupole Time-of-Flight mass spectrometer; |
PMID | |
DOI | |
Citation | Liu J, Fang Y, Cui L, et al. Butyrate emerges as a crucial effector of Zhi-Zi-Chi decoctions to ameliorate depression via multiple pathways of brain-gut axis. Biomed Pharmacother. 2022 May;149:112861. |
Metabolite |
