Study M095
Study name | Xiong ZL 2016 |
Title | Serum metabonomics study of anti-depressive effect of Xiao-Chai-Hu-Tang on rat model of chronic unpredictable mild stress |
Overall design | In this study, the metabolic profiles and potential biomarkers were investigated in a chronic unpredictable mild stress (CUMS) model after treatment with Xiao-Chai-Hu-Tang (XCHT). Metabonomics based on ultra-high performance liquid chromatography coupled with mass spectrometry was used to profile the metabolic fingerprints of serum obtained from a rat model with chronic unpredictable mild stress with and without XCHT treatment. Rats were divided into 4 groups (n = 8 in each group): (1) control group, (2) CUMS group, (3) CUMS + fluoxetine group (stress plus fluoxetine treatment at the dose of 20 mg/kg), and (4) CUMS + XCHT group (stress plus XCHT treatment at the dose of 5 g/kg). The CUMS stress procedure lasted for 6 weeks, and drugs were intragastric administration once per day during the last 4 weeks of the model building period. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test |
Sample size | 32 |
Tissue | Peripheral; Blood; Serum; |
Platform | MS-based; LC-MS: Waters ACQUITY ultra performance liquid chromatography (UPLC) system coupled with Micromass Quattro Micro API mass spectrometer (Waters Corp., Milford, MA, USA); |
PMID | |
DOI | |
Citation | Xiong Z, Yang J, Huang Y, et al. Serum metabonomics study of anti-depressive effect of Xiao-Chai-Hu-Tang on rat model of chronic unpredictable mild stress. J Chromatogr B Analyt Technol Biomed Life Sci 2016;1029-1030:28-35. |
Metabolite | Citric acid; L-Phenylalanine; L-Tryptophan; Hippuric acid; Cholic acid; LysoPC(16:0); |
