Study M053

Study name

Chang X 2015

Title

Role of Bai-Shao towards the antidepressant effect of Chaihu-Shu-Gan-San using metabonomics integrated with chemical fingerprinting

Overall design

In order to explore the role of Bai-Shao towards the antidepressant effect of Chaihu-Shu-Gan-San (CSGS), the metabolic regulation and chemical profiles of CSGS with and without Bai-Shao (QBS) were investigated using metabonomics integrated with chemical fingerprinting. At first, partial least squares regression analysis was applied to characterize the potential biomarkers associated with chronic unpredictable mild stress (CUMS)-induced depression. Differential metabolites were found using the ultra-performance liquid chromatography quadrupole time of flight mass spectrometry (UPLC-Q-TOF/MS) and 1H NMR-based urinary metabonomics. Rats were randomly divided into 3 groups (n = 6 in each group): (1) control group, (2) CUMS group, and (3) CUMS + CSGS group (stress plus CSGS treatment at a dose of equivalent to 31.5 g crude drug/kg). The CUMS stress procedure lasted for 4 weeks, and drugs were administered intragastrically once daily during the model building period.

Study Type

Type1;

Type2;

Data available

Unavailable

Organism

Rat; Wistar rat;

Categories of depression

Animal model; Chronic mild stress model; Chronic mild stress model;

Criteria for depression

Sucrose preference test

Sample size

18

Tissue

Peripheral; Urine; Urine;

Platform

MS-based; LC-MS: Waters Acquity Ultra Performance LC system with SYNAPT HDMS system (Waters Corporation, Milford, MA, USA);

NMR; NMR: Bruker AVIII 600 spectrometer (Bruker Biospin, Germany);

PMID

26540435

DOI

10.1016/j.jchromb.2015.10.007

Citation

Chang X, Jia H, Zhou C, et al. Role of Bai-Shao towards the antidepressant effect of Chaihu-Shu-Gan-San using metabonomics integrated with chemical fingerprinting. J Chromatogr B Analyt Technol Biomed Life Sci 2015;1006:16-29.

Metabolite

myo-Inositol;

N-Acetyl-L-aspartic acid;

L-Cysteine;

L-Glutamic acid;

Hippuric acid;

Pantothenic acid;

Kynurenic acid;

Xanthurenic acid;

Creatinine;

5-Hydroxy-6-methoxyindole glucuronide;

Indole-3-carboxylic acid;

Taurine;

Acetoacetic acid;

2-Phenylethanol glucuronide;

N-Acetylserotonin;

Allantoin;

3-Methyldioxyindole;

p-Cresol glucuronide;

p-Cresol sulfate;