Study M048
Study name | Su ZH 2014 |
Title | Hippocampus and serum metabolomic studies to explore the regulation of Chaihu-Shu-Gan-San on metabolic network disturbances of rats exposed to chronic variable stress |
Overall design | In this study, metabolomic studies of biochemical changes in the hippocampus and serum of chronic variable stress (CVS)-induced depression rats after treatment with Chaihu-Shu-Gan-San (CSGS) were performed using ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS). Rats were randomly divided into 4 groups (n = 8 in each group): (1) control group, (2) CVS group, (3) CVS + CSGS group (stress plus CSGS intragastrically treated at the dose of 2.5 g/kg), and (4) CVS + fluoxetine group (stress plus fluoxetine treated at the dose of 3 mg/kg by oral gavage). The CUMS stress procedure lasted for 4 weeks, and drugs were administered during the model building period. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Wistar rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Not reported |
Sample size | 32 |
Tissue | Central; Brain; Hippocampus; Peripheral; Blood; Serum; |
Platform | MS-based; LC-MS: Waters Acquity Ultra Performance LC system with Q-TOF analyzer in a SYNAPT HDMS system (Waters Corporation, Milford, MA, USA); |
PMID | |
DOI | |
Citation | Su ZH, Jia HM, Zhang HW, et al. Hippocampus and serum metabolomic studies to explore the regulation of Chaihu-Shu-Gan-San on metabolic network disturbances of rats exposed to chronic variable stress. Mol Biosyst 2014;10(3):549-61. |
Metabolite | Myristic acid; N-Acetyl-L-aspartic acid; L-Phenylalanine; L-Tryptophan; Cholic acid; LysoPC(16:0); Phytosphingosine; Spermine; Indoxyl sulfate; Hypoxanthine; Creatine; Niacinamide; Docosahexaenoic acid; Inosine; LysoPC(18:0); (9Z-Hexadecanoyl)-glycerol; Docosanoyl-glycerol; D-Glucurono-6,3-lactone; |
