Study M027

Study name

Jia HM 2013a

Title

Metabonomics combined with UPLC-MS chemical profile for discovery of antidepressant ingredients of a traditional Chinese medicines formula, Chaihu-Shu-Gan-San

Overall design

This study proposed a new strategy for uncovering the active chemical constituents of a traditional Chinese medicines formula, Chaihu-Shu-Gan-San (CSGS). Metabonomics and chemical profile were identified using NMR and UPLC-MS methods to discover the chemical constituents which contribute to the antidepressant effect of CSGS. Rats were randomly divided into 5 groups (n = 8 in each group): (1) control group, (2) CUMS group, (3) CUMS + fluoxetine group (stress plus fluoxetine treatment at the dose of 3.0 mg/kg), (4) CUMS + CSGS group (stress plus CSGS treatment at the dose of equivalent to 31.5 g crude drug/kg body weight), and (5) CUMS + QZ group (stress plus CSGS all herbs except Zhi-Qiao, at the dose of equivalent to 27.0 g crude drug/kg body weight). The CUMS stress procedure lasted for 4 weeks, and drugs were administered during the model building period.

Study Type

Type1;

Type2;

Data available

Unavailable

Organism

Rat; Wistar rat;

Categories of depression

Animal model; Chronic mild stress model; Chronic mild stress model;

Criteria for depression

Sucrose preference test

Sample size

40

Tissue

Peripheral; Urine; Urine;

Platform

NMR; NMR: Bruker AV III 600 spectrometer (Bruker Biospin, Germany);

MS-based; LC-MS: Waters Acquity Ultra Performance LC system with SYNAPT HDMS system (Waters Corporation, Milford, MA, USA);

PMID

23662139

DOI

10.1155/2013/487158

Citation

Jia H, Su Z, Long W, et al. Metabonomics Metabonomics combined with UPLC-MS chemical profile for discovery of antidepressant ingredients of a traditional Chinese medicines formula, Chaihu-Shu-Gan-San. Evid Based Complement Alternat Med 2013;2013:487158.

Metabolite

3-Hydroxybutyric acid;

L-Phenylalanine;

L-Glutamic acid;

L-Serine;

L-Isoleucine;

L-Glutamine;

Hippuric acid;

L-Tyrosine;

L-Kynurenine;

Kynurenic acid;

DOPA;

Pyruvic acid;

Creatine;

Histamine;

alpha-D-Glucose;

5-Methoxytryptamine;

Tryptophanol;

Isobutyrylglycine;

Acetoacetic acid;

3-Hydroxyhippuric acid;

2-Aminomuconic acid semialdehyde;

2-Amino-3-carboxymuconic acid semialdehyde;

Dopamine;

2-Phenylethanol glucuronide;

Lipid;

5-Aminoimidazole-4-carboxamide;

Glyceric acid 1,3-biphosphate;

N-Acetylserotonin;