Study M012
Study name | Su ZH 2011 |
Title | Urinary metabonomics study of anti-depressive effect of Chaihu-Shu-Gan-San on an experimental model of depression induced by chronic variable stress in rats |
Overall design | In this study, metabonomics based on ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) was used to profile the metabolic fingerprints of urine obtained from chronic variable stress (CVS)-induced depression model in rats with and without Chaihu-Shu-Gan-San (CSGS) treatment. Rats were randomly divided into 4 groups (n = 8 in each group): (1) control group, (2) CVS group, (3) CVS + CSGS group (stress plus CSGS intragastrically treated at the dose of 2.5 g/kg), and (4) CVS + fluoxetine group (stress plus fluoxetine treated at the dose of 3 mg/kg by oral gavage). The CVS stress procedure lasted for 4 weeks, and drugs were administered intragastrically during the model building period. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Wistar rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test |
Sample size | 32 |
Tissue | Peripheral; Urine; Urine; |
Platform | MS-based; LC-MS: Waters Acquity Ultra Performance LC system with Q-TOF analyzer in SYNAPT HDMS system (Waters Corporation, Milford, MA, USA); |
PMID | |
DOI | |
Citation | Su ZH, Li SQ, Zou GA, et al. Urinary metabonomics study of anti-depressive effect of Chaihu-Shu-Gan-San on an experimental model of depression induced by chronic variable stress in rats. J Pharm Biomed Anal 2011;55(3):533-9. |
Metabolite | L-Proline; Pantothenic acid; Kynurenic acid; Xanthurenic acid; 3-Methoxytyrosine; 2,8-Dihydroxyquinoline-beta-D-glucuronide; |
