Study M985
Study name | Chen Y 2022 |
Title | Indole acetic acid exerts anti-depressive effects on an animal model of chronic mild stress |
Overall design | In this study, a mouse model of chronic unpredictable mild stress (CUMS) was used to assess the effects of indole acetic acid administration. C57BL/6J mice were divided into the following 3 groups (n = 6-8 in each group): (1) control group (no stress plus saline injection), (2) CUMS group (stressor plus saline injection), and (3) CUMS + indole acetic acid group (stressor plus indole acetic acid treatment at the dose of 50 mg/kg). The CUMS stress procedure lasted for 5 weeks, and indole acetic acid was intraperitoneally injected for 5 weeks during stress. Serotonin, 5-hydroxytryptophan, and dopamine levels in the plasma, colon content and hippocampus were measured by UPLC-MS. The analysis of short-chain fatty acids in the caecum was conducted by GC-MS. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Mouse; C57BL/6J mouse; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Forced swimming test |
Sample size | 20 |
Tissue | Peripheral; Blood; Plasma; Peripheral; Gut; Colon; Central; Brain; Hippocampus; Peripheral; Gut; Cecum; |
Platform | MS-based; LC-MS: DIONEX UltiMate 3000 HPLC with Q-Exactive mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA); MS-based; GC-MS: not reported; |
PMID | |
DOI | |
Citation | Chen Y, Tian P, Wang Z, et al. Indole acetic acid exerts anti-depressive effects on an animal model of chronic mild stress. Nutrients 2022;14, 5019. |
Metabolite | Indoleacetic acid; Serotonin; |
