Study M964
Study name | Tung TH 2022 |
Title | N-3 PUFA ameliorates the gut microbiota, bile acid profiles, and neuropsychiatric behaviours in a rat model of geriatric depression |
Overall design | The aim of this study was to investigate whether different dietary oil interventions affected dysbiosis in a chronic unpredictable mild stress (CUMS)- and D-galactose-induced ageing model and explored the possible mechanisms underlying the lipids on intestinal dysbiosis and neuropsychiatric behaviours induced by CUMS. Sprague-Dawley rats were divided into the following 4 groups: (1) control group (normal diet), (2) D-galactose + CUMS group (normal diet, with D-galactose injections), (3) D-galactose + CUMS + imipramine group (normal diet, with D-galactose and imipramine injections), and (4) D-galactose + CUMS + fish oil diet group (normal diet, with D-galactose injections and fish oil diet). The experiment was conducted over 32 weeks. The D-galactose injections (s.c. 500 mg/kg) were administered continually during the experimental period. The CUMS protocol was applied from week 16 until the end of the experiment. The fish oil and corn oil diets were provided from week 20 until the end of the experiment. Imipramine was administered in the drinking water (20 mg/kg) daily from week 26 to week 32. After experiment, bile acid profiles of rodent faecal samples were measured by LC-MS (n = 6/ group). |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Other animal model; Other animal model; |
Criteria for depression | Forced swimming test |
Sample size | 24 |
Tissue | Peripheral; Faece; Faece; |
Platform | MS-based; LC-MS: Waters ultra-high-performance liquid chromatography system coupled with Waters Xevo tandem quadrupole mass spectrometer (TQ-S MS, Waters, Milford, MA, USA); |
PMID | |
DOI | |
Citation | Tung TH, Chen YC, Lin YT, et al. N-3 PUFA ameliorates the gut microbiota, bile acid profiles, and neuropsychiatric behaviours in a rat model of geriatric depression. Biomedicines. 2022 Jul 4;10(7):1594. |
Metabolite | Cholic acid; Hyodeoxycholic acid; Ursodeoxycholic acid; Nutriacholic acid; Taurinehyodeoxycholic acid; Taurine-ursodeoxycholic acid/Ursodeoxycholic acid ratio; |
