Study M943
Study name | Gu XY 2022 |
Title | Integrated network pharmacology and hepatic metabolomics to reveal the mechanism of Acanthopanax senticosus against major depressive disorder |
Overall design | In this study, hepatic metabolomics based on gas chromatography-mass spectrometry (GC-MS) combined with network pharmacology was applied to find biomarkers, related pathways, hub targets, and key compounds of Acanthopanax senticosus (Rupr. et Maxim.) Harms (ASH) in the chronic unpredictable mild stress (CUMS) model of depression. ICR mice were divided into the following 6 groups (n = 6 in each group): (1) control group, (2) CUMS group, (3) CUMS + fluoxetine group (stressor plus fluoxetine treatment at the dose of 10 mg/kg), (4) CUMS + low dose of Acanthopanax senticosus Harms group (stressor plus Acanthopanax senticosus Harms treatment at the dose of 100 mg/kg), (5) CUMS + middle dose of Acanthopanax senticosus Harms group (stressor plus Acanthopanax senticosus Harms treatment at the dose of 200 mg/kg), and (6) CUMS + high dose of Acanthopanax senticosus Harms group (stressor plus Acanthopanax senticosus Harms treatment at the dose of 400 mg/kg). The CUMS stress procedure lasted for 6 weeks, and drugs were administered via intragastric once per day during the model building period. Liver samples were used for metabonomic analysis. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Mouse; ICR mouse; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Forced swimming test, tail suspension test |
Sample size | 36 |
Tissue | Peripheral; Liver; Liver; |
Platform | MS-based; GC-MS: 6890N-5975B (Agilent; United States); |
PMID | |
DOI | |
Citation | Gu X, Zhang G, Wang Q, et al. Integrated network pharmacology and hepatic metabolomics to reveal the mechanism of Acanthopanax senticosus against major depressive disorder. Front Cell Dev Biol. 2022 Aug 5;10:900637. |
Metabolite | myo-Inositol; Stearic acid; L-Valine; L-Leucine; L-Proline; Glycine; L-Serine; L-Threonine; L-Isoleucine; L-Alanine; L-Glutamine; L-Aspartic acid; D-Glucose; Gluconic acid; |
