Study M940
Study name | Chen JJ 2022 |
Title | Integration of non-targeted metabolomics and targeted quantitative analysis to elucidate the synergistic antidepressant effect of Bupleurum chinense DC-Paeonia lactiflora Pall herb pair by regulating purine metabolism |
Overall design | The aim of this study was to investigate the compatibility mechanism of Chaihu and Baishao for treating depression in the chronic unpredictable mild stress (CUMS) model of depression. Sprague-Dawley rats were divided into the following 4 groups: (1) control group, (2) CUMS group, (3) CUMS + Chaihu group (stressor plus Chaihu treatment at the dose of 7.5 crude drug/kg), and (4) CUMS + Chaihu-Baishao herb pair group (stressor plus Chaihu-Baishao herb pair treatment at the dose of 7.5 crude drug/kg). The CUMS stress procedure lasted for 4 weeks, and drugs were administered via intragastric once per day during the model building period. Cortex metabolomics based on ultra-high-performance liquid chromatography combined with quadrupole orbitrap mass spectrometry was used to discover the metabolic pathway that was synergistically regulated by Chaihu-Baishao herb pair. Based on the results of untargeted metabolomics analysis, metabolites were quantitatively validated by UPLC-MS/MS combined with the MRM mode in the crucial metabolic pathway. Six samples per group were used for both untargeted and targeted metabonomic analysis. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test, forced swimming test |
Sample size | 24 |
Tissue | Central; Brain; Cerebral cortex; |
Platform | MS-based; LC-MS: Agilent 1290 series UPLC system (Agilent Technologies, CA, United States) with AB SCIEX API 3200MD; MS-based; LC-MS: Thermo-Fisher Dionex UltiMate 3000 UPLC-Q Exactive Orbitrap-MS; |
PMID | |
DOI | |
Citation | Chen J, Li T, Qin X, et al. Integration of non-targeted metabolomics and targeted quantitative analysis to elucidate the synergistic antidepressant effect of Bupleurum chinense DC-Paeonia lactiflora Pall herb pair by regulating purine metabolism. Front Pharmacol. 2022 Jun 30;13:900459. |
Metabolite | Citric acid; Pyroglutamic acid; Phosphoserine; N-Acetyl-L-aspartic acid; L-Phenylalanine; L-Glutamic acid; Choline; Pantothenic acid; Hypoxanthine; Xanthine; Taurine; Inosine; Xanthosine; Adenosine; Guanosine monophosphate; Adenosine monophosphate; Inosinic acid; Adenine; Nicotinic acid; Guanosine; L-Palmitoylcarnitine; gamma-Glutamylglutamic acid; Guanine; 5-Dehydro-4-deoxy-6-D-glucuronate; |
