Study M936

Study name

Liu XE 2022

Title

Comparative analysis of gut microbiota and fecal metabolome features among multiple depressive animal models

Overall design

The aim of the present study was to investigate the fecal metabolome in four stress models of depression. Sprague-Dawley rats were exposed to chronic unpredictable mild stress (CUMS), inescapable foot-shock stress (learned helplessness, LH), repeated restraint stress (chronic restraint stress, CRS) and resident-intruder stress (social defeat, SD) to mimic different types of stress in humans. In Experiment 1, rats were divided into (1) control group and (2) CUMS group. In Experiment 2, rats were divided into (3) control group and (4) LH group. In Experiment 3, rats were divided into (5) control group and (6) CRS group. In Experiment 4, rats were divided into (7) control group and (8) SD group. All stress procedures lasted for 3 weeks. Only susceptible rats were used for fecal metabolomics analysis. Then LC-MS non-targeted metabolomics analysis was employed to identify the alternations of fecal metabolites (n =6/ per group).

Study Type

Type1;

Data available

Unavailable

Organism

Rat; Sprague-Dawley rat;

Categories of depression

Animal model; Chronic restraint stress model; Chronic restraint stress model;

Animal model; Learned helplessness model; Learned helplessness model;

Animal model; Social defeat model; Social defeat model;

Animal model; Chronic mild stress model; Chronic mild stress model;

Criteria for depression

Sucrose preference test, forced swimming test

Sample size

48

Tissue

Peripheral; Faece; Faece;

Platform

MS-based; LC-MS: UHPLC-Q-Exactive HF-X;

PMID

35780963

DOI

10.1016/j.jad.2022.06.088

Citation

Liu X, Li X, Teng T, et al. Comparative analysis of gut microbiota and fecal metabolome features among multiple depressive animal models. Journal of Affective Disorders. 2022. July 1.

Metabolite

L-Glutamic acid;

Cholic acid;

Phytosphingosine;

N-Acetyl-L-glutamic acid;

Glycocholic acid;

Deoxycholic acid;

Sphingosine 1-phosphate;

LysoPC(20:0/0:0);

LysoPC(20:2(11Z,14Z)/0:0);

PC(14:0/20:1(11Z));

Sphingomyelin;

Calcitriol;

Ergocalciferol;

Cholesterol ester;

PC(16:0/20:3(8Z,11Z,14Z));

Chondroitin 4-sulfate;

Amprotropine;