Study name | Liu XE 2022 |
Title | Comparative analysis of gut microbiota and fecal metabolome features among multiple depressive animal models |
Overall design | The aim of the present study was to investigate the fecal metabolome in four stress models of depression. Sprague-Dawley rats were exposed to chronic unpredictable mild stress (CUMS), inescapable foot-shock stress (learned helplessness, LH), repeated restraint stress (chronic restraint stress, CRS) and resident-intruder stress (social defeat, SD) to mimic different types of stress in humans. In Experiment 1, rats were divided into (1) control group and (2) CUMS group. In Experiment 2, rats were divided into (3) control group and (4) LH group. In Experiment 3, rats were divided into (5) control group and (6) CRS group. In Experiment 4, rats were divided into (7) control group and (8) SD group. All stress procedures lasted for 3 weeks. Only susceptible rats were used for fecal metabolomics analysis. Then LC-MS non-targeted metabolomics analysis was employed to identify the alternations of fecal metabolites (n =6/ per group). |
Type1; | |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic restraint stress model; Chronic restraint stress model; Animal model; Learned helplessness model; Learned helplessness model; Animal model; Social defeat model; Social defeat model; Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test, forced swimming test |
Sample size | 48 |
Tissue | Peripheral; Faece; Faece; |
Platform | MS-based; LC-MS: UHPLC-Q-Exactive HF-X; |
PMID | |
DOI | |
Citation | Liu X, Li X, Teng T, et al. Comparative analysis of gut microbiota and fecal metabolome features among multiple depressive animal models. Journal of Affective Disorders. 2022. July 1. |
Metabolite |