Study M890
Study name | Feng JQ 2021 |
Title | Simultaneous analysis of the metabolome and lipidome using polarity partition two-dimensional liquid chromatography-mass spectrometry |
Overall design | In this study, a heart-cutting two-dimensional liquid chromatography-mass spectrometry (2D-LC-MS) method-based polarity partition was established to analyze both the metabolome and lipidome in a single run. The feasibility of the 2D-LC-MS system was demonstrated by simultaneous analysis of the metabolome and lipidome in plasma samples of chronic unpredictable mild stress (CUMS) rat model of depression. Sprague-Dawley rats were divided into the following 2 groups (n = 12 in each group): (1) control group, and (2) CUMS group. The CUMS stress procedure lasted for 4 weeks. |
Study Type | Type1; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Not reported |
Sample size | 24 |
Tissue | Peripheral; Blood; Plasma; |
Platform | MS-based; LC-MS: Nexera X2 (Shimadzu, Japan) with Q-TOF-MS (LC−MS 9030); |
PMID | |
DOI | |
Citation | Feng J, Zhong Q, Kuang J, et al. Simultaneous analysis of the metabolome and lipidome using polarity partition two-dimensional liquid chromatography-mass spectrometry. Anal Chem. 2021 Nov 16;93(45):15192-15199. |
Metabolite | Oleic acid; Stearic acid; L-Proline; L-Glutamic acid; LysoPC(16:0); Uric acid; LysoPC(18:0); L-Carnitine; LysoPC(18:1); LysoPC(18:2); LysoPC(20:1); PC(32:1); PC(36:4); PC(37:4); PC(38:6); TG(60:12); PE(34:2); PC(36:2); PC(35:4); PE(38:4); Proline betaine; LysoPC(20:4); LysoPC(20:5); PC(40:7); DG(34:1); PC(42:10); TG(54:3); LysoPC(17:0/0:0); TG(58:10); TG(54:7); TG(58:8); LysoPC(20:2); |
