Study M888
Study name | Chevalier G 2020b |
Title | Effect of gut microbiota on depressive-like behaviors in mice is mediated by the endocannabinoid system |
Overall design | The aim of this study was to explore a causative role of the gut microbiota in stress-induced depressive behaviors in the chronic unpredictable mild stress (CUMS) model of depression and fecal microbiota transfer (FMT) from stressed donors to naive mice. C57BL/6J mice were divided into control group and CUMS group. The CUMS stress procedure lasted for 8 weeks. Fresh fecal pellets were collected directly from the rectum of control and CUMS mice after stress. Recipient mice were given a combination of antibiotics, then colonized via two rounds of oral gavage with microbiota, separated 3 days apart, and kept in separate sterile isolators. Recipient mice were divided into the following 4 groups: (1) control microbiota group (antibiotics-treated mice colonized with microbiota from control mice), (2) CUMS microbiota group (antibiotics-treated mice colonized with microbiota from CUMS mice), (3) CUMS microbiota + arachidonic acid group (antibiotics-treated mice colonized with microbiota from CUMS mice, with 8 mg of arachidonic acid/mouse/day, every 2 days through oral gavage), and (4) CUMS microbiota + L. plantarum Lp group (antibiotics-treated mice colonized with microbiota from CUMS mice, with L. plantarum Lp, 2 x 10*8 CFU, 5 days a week through oral feeding). Recipient animals were left in the isolators for 8 weeks before any analysis. The hippocampal content of 2-arachidonoylglycerol was determined using isotope-dilution liquid chromatography-electrospray ionization tandem mass spectrometry (n = 5/per group). Hippocampal polyunsaturated fatty acid and anandamide levels were detected by supercritical fluid chromatography coupled to mass spectrometry (n = 5/per group). |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Mouse; C57BL/6J mouse; |
Categories of depression | Animal model; Other animal model; Other animal model; |
Criteria for depression | Tail suspension test, forced swimming test |
Sample size | 20 |
Tissue | Central; Brain; Hippocampus; Peripheral; Blood; Serum; |
Platform | MS-based; GC-MS: Thermo-Finnigan Trace DSQ fast-scanning single-quadrupole mass spectrometer; MS-based; LC-MS: SFC (Acquity UPC2, Waters, France) with Xevo TQ-MS triple quadrupole mass spectrometer (Waters, USA); MS-based; LC-MS: Waters ACQUITY UPLC and Thermo-Finnigan LTQ mass spectrometer; |
PMID | |
DOI | |
Citation | Chevalier G, Siopi E, Guenin-Mace L, et al. Effect of gut microbiota on depressive-like behaviors in mice is mediated by the endocannabinoid system. Nat Commun. 2020 Dec 11;11(1):6363. |
Metabolite | Arachidonic acid; Linoelaidylcarnitine; Oleoylcarnitine; |
