Study M887
Study name | Chevalier G 2020a |
Title | Effect of gut microbiota on depressive-like behaviors in mice is mediated by the endocannabinoid system |
Overall design | The aim of this study was to explore a causative role of the gut microbiota in stress-induced depressive behaviors in the chronic unpredictable mild stress (CUMS) model of depression and fecal microbiota transfer (FMT) from stressed donors to naive mice. C57BL/6J mice were divided into the following 2 groups: (1) control group, and (2) CUMS group. The CUMS stress procedure lasted for 8 weeks. The hippocampal content of 2-arachidonoylglycerol was determined using isotope-dilution liquid chromatography-electrospray ionization tandem mass spectrometry (n = 5/per group). Hippocampal polyunsaturated fatty acid and anandamide levels were detected by supercritical fluid chromatography coupled to mass spectrometry (n = 4/per group). Serum samples were then extracted and analyzed on GC/MS, LC/MS, and LC/MS/MS platforms (n = 5/per group). |
Study Type | Type1; |
Data available | Unavailable |
Organism | Mouse; C57BL/6J mouse; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Tail suspension test, forced swimming test |
Sample size | 10 |
Tissue | Peripheral; Blood; Serum; Central; Brain; Hippocampus; |
Platform | MS-based; LC-MS: SFC (Acquity UPC2, Waters, France) with Xevo TQ-MS triple quadrupole mass spectrometer (Waters, USA); MS-based; LC-MS: Waters ACQUITY UPLC and Thermo-Finnigan LTQ mass spectrometer; MS-based; GC-MS: Thermo-Finnigan Trace DSQ fast-scanning single-quadrupole mass spectrometer; |
PMID | |
DOI | |
Citation | Chevalier G, Siopi E, Guenin-Mace L, et al. Effect of gut microbiota on depressive-like behaviors in mice is mediated by the endocannabinoid system. Nat Commun. 2020 Dec 11;11(1):6363. |
Metabolite |
