Study M844
Study name | Tian JS 2022 |
Title | Stable isotope-resolved metabolomics studies on corticosteroid-induced PC12 cells: a strategy for evaluating glucose catabolism in an in vitro model of depression |
Overall design | The aim of this study was to research the glucose catabolism of the corticosterone-induced PC12 cell damage model of depression by combining traditional metabolomics technology with stable isotope-resolved metabolomics. PC12 cells were divided into the no treatment control group (control group) and the corticosterone group (CORT group). The control group of PC12 cells was added 10 mL of 1640 medium, and the corticosterone group was added 10 mL of 1640 medium containing 250 uM corticosterone. After 24 h of incubation, three samples per group were used for metabonomic analysis. |
Study Type | Type1; |
Data available | Unavailable |
Organism | Rat; Rat; |
Categories of depression | Animal model; Other animal model; Other animal model; |
Criteria for depression | Not reported |
Sample size | 6 |
Tissue | Central; Brain; PC12 cell; |
Platform | MS-based; LC-MS: Thermo Dionex UltiMate 3000 high-performance liquid chromatograph with Thermo Q Exactive HF hybrid quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific., Germany); |
PMID | |
DOI | |
Citation | Tian JS, Wu WZ, Liu SB, et al. Stable isotope-resolved metabolomics studies on corticosteroid-induced PC12 cells: a strategy for evaluating glucose catabolism in an in vitro model of depression. J Proteome Res. 2022 Mar 4;21(3):788-797. |
Metabolite | N-Acetyl-L-aspartic acid; L-Tryptophan; L-Leucine; L-Proline; L-Glutamic acid; L-Serine; L-Methionine; L-Lactic acid; L-Alanine; L-Aspartic acid; D-Glucose; Hypoxanthine; Fumaric acid; Taurine; Pyruvic acid; 4-Hydroxybutyric acid; |
