Study M827
Study name | Zhang C 2021 |
Title | Urinary metabonomics study of anti-depressive mechanisms of Millettia speciosa Champ on rats with chronic unpredictable mild stress-induced depression |
Overall design | In this study, urine metabolomics based on ultra-performance liquid chromatography/quadrupole time of flight mass spectrometry combined with pharmacodynamics was used to explore the pathogenesis of chronic unpredictable mild stress (CUMS)-induced depression and the antidepressant effects of Millettia speciosa Champ. Sprague-Dawley rats were divided into the following 6 groups (n = 6 per group): (1) control group, (2) CUMS group, (3) CUMS + high dose of Millettia speciosa Champ group (stressor plus Millettia speciosa Champ treatment at the dose of 14 g/kg), (4) CUMS + middle dose of Millettia speciosa Champ group (stressor plus Millettia speciosa Champ treatment at the dose of 7 g/kg), (5) CUMS + low dose of Millettia speciosa Champ group (stressor plus Millettia speciosa Champ treatment at the dose of 3.5 g/kg), and (6) CUMS + fluoxetine group (stressor plus fluoxetine treatment at the dose of 2.1 mg/kg). Urine samples (n = 5-6/group) were used for metabonomic analysis. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test |
Sample size | 35 |
Tissue | Peripheral; Urine; Urine; |
Platform | MS-based; LC-MS: Waters Acquity Ultra Performance LC system (Waters Corporation, Milford, MA, USA) with Xevo G2-S Q-TOF instrument (ACQUITY, WATERS, Milford, USA); |
PMID | |
DOI | |
Citation | Zhang C, Mo YY, Feng SS, et al. Urinary metabonomics study of anti-depressive mechanisms of Millettia speciosa Champ on rats with chronic unpredictable mild stress-induced depression. J Pharm Biomed Anal. 2021 Oct 25;205:114338. |
Metabolite | Glutaric acid; L-Isoleucine; |
