Study M811
Study name | Topuz RD 2021 |
Title | The role of endocannabinoid system and TRPV1 receptors in the antidepressant and anxiolytic effects of dipyrone in chronic unpredictable mild stress in mice |
Overall design | The aim of this study was to investigate endocannabinoid levels in the amygdala in chronic unpredictable mild stress (CUMS) model in mice. Balb/c mice were divided into the following 5 groups (n = 8 in each group): (1) control group, (2) CUMS group, (3) CUMS + low dose of dipyrone group (stressor plus dipyrone treatment at the dose of 150 mg/kg), (4) CUMS + middle dose of dipyrone group (stressor plus dipyrone treatment at the dose of 300 mg/kg), and (5) CUMS + high dose of dipyrone group (stressor plus dipyrone treatment at the dose of 600 mg/kg). The CUMS stress procedure lasted for 6 weeks, and all drugs were administered 30 min before the behavioral tests on testing days. Endocannabinoid and N-acylethanolamines levels were measured by LC-MS/MS in amygdala. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Mouse; Balb/c mouse; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test, forced swimming test |
Sample size | 40 |
Tissue | Central; Brain; Amygdala; |
Platform | MS-based; LC-MS: Agilent LC-MS (Agilent Technologies, Waldbronn, Germany) 6460 triple quadruple mass spectrometer; |
PMID | |
DOI | |
Citation | Topuz RD, Cetinkaya MZ, Erumit D, et al. The role of endocannabinoid system and TRPV1 receptors in the antidepressant and anxiolytic effects of dipyrone in chronic unpredictable mild stress in mice. Eur J Pharmacol. 2021 Oct 5;908:174315. |
Metabolite |
