Study M800
Study name | Tkachev A 2021 |
Title | Long-term fluoxetine administration causes substantial lipidome alteration of the juvenile macaque brain |
Overall design | The aim of this study was to assess residual effects of two-year fluoxetine administration on the expression of genes and abundance of lipids and polar metabolites in the prelimbic cortex of 10 treated and 11 control macaques. Rhesus monkeys were divided into the following 2 groups: (1) control group, and (2) fluoxetine group. For the first 11 months fluoxetine (1.6 mg/kg) was administered daily. After analysis of steady state serum levels, the dose was adjusted to 2.4 mg/kg for the remainder of the dosing period (~1 year) followed by a four-week tapered discontinuation period and a two-week washout at the onset of the post-dosing period. Alterations of gene expression, polar metabolite, and lipid abundance in the prelimbic cortex of macaques treated with fluoxetine were assessed using RNA-sequencing, Fourier-transform ion cyclotron resonance mass spectrometry, and high precision mass spectrometry coupled with liquid chromatography, respectively. |
Study Type | Type3; |
Data available | |
Organism | Non-human primate; Rhesus monkey; |
Categories of depression | Healthy individuals; Healthy individuals; Healthy individuals; |
Criteria for depression | Not reported |
Sample size | 21 |
Tissue | Central; Brain; Prelimbic cortex; |
Platform | MS-based; FT-ICR-MS: 12 T solariX FT-ICR mass spectrometer (Bruker Daltonics, Billerica, MA, USA); MS-based; LC-MS: Waters Acquity I-class UPLC system (Waters, Manchester, UK) and a Q Exactive Orbitrap mass spectrometer (Thermo Fisher Scientific, USA); |
PMID | |
DOI | |
Citation | Tkachev A, Stekolshchikova E, Bobrovskiy DM, et al. Long-term fluoxetine administration causes substantial lipidome alteration of the juvenile macaque brain. Int J Mol Sci. 2021 Jul 28;22(15):8089. |
Metabolite | Arachidonic acid; Docosahexaenoic acid; O-Phosphoethanolamine; Hexosylceramide O2; hexosylceramide O3; |
