Study M789
Study name | Liu DY 2021a |
Title | A high coverage pseudotargeted lipidomics method based on three-phase liquid extraction and segment data-dependent acquisition using UHPLC-MS/MS with application to a study of depression rats |
Overall design | This study proposed a comprehensive pseudotargeted lipidomics method based on three-phase liquid extraction (3PLE) and segment data-dependent acquisition (SDDA). The established pseudotargeted method was applied to the plasma lipid profiling from the chronic unpredictable mild stress (CUMS) rat model of depression. Sprague-Dawley rats were divided into the following 4 groups (n = 8 in each group): (1) control group, (2) CUMS group, (3) CUMS + liquiritin group (stressor plus liquiritin suspension treatment at the dose of 20 mg/kg), and (4) CUMS + fluoxetine group (stressor plus fluoxetine treatment at the dose of 10 mg/kg). The CUMS stress procedure lasted for 4 weeks, and drugs were administered via intragastric once per day during the model building period. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Not reported |
Sample size | 32 |
Tissue | Peripheral; Blood; Plasma; |
Platform | MS-based; LC-MS: ACQUITY UHPLC I-Class (Waters, USA) system with SYNAPT-G2S Q-TOF mass spectrometer (Waters, USA); MS-based; LC-MS: UHPLC-TQ MS system (Shimadzu, Japan) with LCMS-8060 mass spectrometer; |
PMID | |
DOI | |
Citation | Liu D, Yang J, Jin W, et al. A high coverage pseudotargeted lipidomics method based on three-phase liquid extraction and segment data-dependent acquisition using UHPLC-MS/MS with application to a study of depression rats. Anal Bioanal Chem. 2021 Jun;413(15):3975-3986. |
Metabolite | Arachidonic acid; LysoPC(16:0); Phytosphingosine; LysoPC(18:1); LysoPC(18:2); PC(36:4); PC(37:4); PC(38:4); PC(38:5); PC(38:6); PC(40:6); LysoPC(20:4); SM(d42:2); PC(38:2); |
