Study M744
Study name | Shan BX 2020b |
Title | Gut microbiome-derived lactate promotes to anxiety-like behaviors through GPR81 receptor-mediated lipid metabolism pathway |
Overall design | The aim of this study was to investigate the direct links between the gut microbiome and metabolome and their impact on anxiety, behavior in chronic unpredictable stress (CUS) rats using the combination of microbiome and metabolome analyses. Sprague-Dawley rats were divided into the following 2 groups: (1) control group, and (2) CUS group. The CUS stress procedure lasted for 30 days. Then fresh fecal samples were collected 1 h after 30 days of CUS. Then rats were fed freely with water containing antibiotics as microbiota-depleted. Fecal samples from randomly chosen CUS group and control group were used to colonize the guts of microbiota-depleted rat. Hippocampal metabolome profile of the CUS transplant group and the control transplant group were compared using LC-MS (n = 6 per group). |
Study Type | Type1; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Other animal model; Other animal model; |
Criteria for depression | Forced swimming test, tail suspension test |
Sample size | 12 |
Tissue | Central; Brain; Hippocampus; |
Platform | MS-based; LC-MS: UltiMate 3000 UHPLC system (Thermo Scientific, San Jose, CA, USA) with orbitrap mass spectrometer (LTQ ORBITRAP VELOS PRO, Thermo Fisher Scientific, San Jose, CA, USA); |
PMID | |
DOI | |
Citation | Shan B, Ai Z, Zeng S, et al. Gut microbiome-derived lactate promotes to anxiety-like behaviors through GPR81 receptor-mediated lipid metabolism pathway. Psychoneuroendocrinology. 2020 Jul;117:104699. |
Metabolite | Arachidonic acid; LysoPC(16:0); Cholesterol; Corticosterone; Sphinganine; 5-Methylcytosine; L-Histidinol; PE(20:2(11Z,14Z)/P-18:1(11Z)); Glycitein; Ethyl hexadecanoate; 25-Hydroxycholesterol; PC(18:0/18:0); PC(18:3(6Z,9Z,12Z)/20:1(11Z)); threo-Syringoylglycerol; Dihydroceramide; CE(17:1); Dihydrofukinolide; PA(8:0/10:0); |
