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Study M728

Study name

Deng YY 2021a

Title

Involvement of the microbiota-gut-brain axis in chronic restraint stress: disturbances of the kynurenine metabolic pathway in both the gut and brain

Overall design

In this study, a murine model of chronic restraint stress (CRS) was established to investigate the metabolic signaling of tryptophan neurotransmission at the intestinal and central levels in depression. C57BL/6 J mice were divided into the following 4 groups: (1) control group, (2) CRS group, (3) citalopram group (citalopram treatment at the dose of 10 mg/kg), and (4) CRS + citalopram group (CRS plus citalopram treatment at the dose of 10 mg/kg). The CRS stress procedure lasted for 2 weeks, and citalopram was administered via intraperitoneally for 3 weeks after stress. Brain (prefrontal cortex and hippocampus), gut (duodenum, jejunum, ileum, colon and cecum) and serum samples were used to measure neurotransmitter levels using LC-MS (n = 10 per group).

Study Type

Type1;

Type2;

Type3;

Data available

Unavailable

Organism

Mouse; C57BL/6 J mouse;

Categories of depression

Animal model; Chronic restraint stress model; Chronic restraint stress model;

Healthy individuals; Healthy individuals; Healthy individuals;

Criteria for depression

Sucrose preference test, forced swimming test

Sample size

40

Tissue

Central; Brain; Prefrontal cortex;

Peripheral; Gut; Jejunum;

Central; Brain; Hippocampus;

Peripheral; Blood; Serum;

Peripheral; Gut; Duodenum;

Peripheral; Gut; Ileum;

Peripheral; Gut; Colon;

Peripheral; Gut; Cecum;

Platform

MS-based; LC-MS: UHPLC Ultimate 3000 system coupled with a Q Exactive hybrid quadrupole-orbitrap mass spectrometer;

PMID

33535879

DOI

10.1080/19490976.2020.1869501

Citation

Deng Y, Zhou M, Wang J, et al. Involvement of the microbiota-gut-brain axis in chronic restraint stress: disturbances of the kynurenine metabolic pathway in both the gut and brain. Gut Microbes. 2021 Jan-Dec;13(1):1-16.

Metabolite

L-Tryptophan;

5-Hydroxyindoleacetic acid;

L-Kynurenine;

Serotonin;

5-HIAA/5-HT ratio;

3-Hydroxyanthranilic acid;

Kynurenine/Tryptophan ratio;

Serotonin/Tryptophan ratio;