Study M728
Study name | Deng YY 2021a |
Title | Involvement of the microbiota-gut-brain axis in chronic restraint stress: disturbances of the kynurenine metabolic pathway in both the gut and brain |
Overall design | In this study, a murine model of chronic restraint stress (CRS) was established to investigate the metabolic signaling of tryptophan neurotransmission at the intestinal and central levels in depression. C57BL/6 J mice were divided into the following 4 groups: (1) control group, (2) CRS group, (3) citalopram group (citalopram treatment at the dose of 10 mg/kg), and (4) CRS + citalopram group (CRS plus citalopram treatment at the dose of 10 mg/kg). The CRS stress procedure lasted for 2 weeks, and citalopram was administered via intraperitoneally for 3 weeks after stress. Brain (prefrontal cortex and hippocampus), gut (duodenum, jejunum, ileum, colon and cecum) and serum samples were used to measure neurotransmitter levels using LC-MS (n = 10 per group). |
Study Type | Type1; Type2; Type3; |
Data available | Unavailable |
Organism | Mouse; C57BL/6 J mouse; |
Categories of depression | Animal model; Chronic restraint stress model; Chronic restraint stress model; Healthy individuals; Healthy individuals; Healthy individuals; |
Criteria for depression | Sucrose preference test, forced swimming test |
Sample size | 40 |
Tissue | Central; Brain; Prefrontal cortex; Peripheral; Gut; Jejunum; Central; Brain; Hippocampus; Peripheral; Blood; Serum; Peripheral; Gut; Duodenum; Peripheral; Gut; Ileum; Peripheral; Gut; Colon; Peripheral; Gut; Cecum; |
Platform | MS-based; LC-MS: UHPLC Ultimate 3000 system coupled with a Q Exactive hybrid quadrupole-orbitrap mass spectrometer; |
PMID | |
DOI | |
Citation | Deng Y, Zhou M, Wang J, et al. Involvement of the microbiota-gut-brain axis in chronic restraint stress: disturbances of the kynurenine metabolic pathway in both the gut and brain. Gut Microbes. 2021 Jan-Dec;13(1):1-16. |
Metabolite | L-Tryptophan; 5-Hydroxyindoleacetic acid; L-Kynurenine; |
