Study M721

Study name

Siopi E 2020a

Title

Changes in gut microbiota by chronic stress impair the efficacy of fluoxetine

Overall design

In this study, the authors used the chronic unpredictable mild stress (CUMS) model of depression to study the effect of gut microbiota on the antidepressant and neurogenic effects of fluoxetine. C57BL/6J mice were divided into the following 3 groups: (1) control group, (2) CUMS group, and (3) CUMS + fluoxetine group (stressor plus fluoxetine treatment at the dose of 18 mg/kg). The CUMS stress procedure lasted for 8 weeks, and drugs were administered via water during the last 4 weeks of the model building period. Five serum and hippocampus samples per group were used for metabonomic analysis.

Study Type

Type1;

Type2;

Data available

Unavailable

Organism

Mouse; C57BL/6J mouse;

Categories of depression

Animal model; Chronic mild stress model; Chronic mild stress model;

Criteria for depression

Forced swimming test, tail suspension test

Sample size

15

Tissue

Central; Brain; Hippocampus;

Peripheral; Blood; Serum;

Platform

MS-based; GC-MS: Thermo-Finnigan Trace DSQ fast-scanning single-quadrupole mass spectrometer;

MS-based; LC-MS: Waters ACQUITY UPLC and Thermo-Finnigan LTQ mass spectrometer;

PMID

32187541

DOI

10.1016/j.celrep.2020.02.099

Citation

Siopi E, Chevalier G, Katsimpardi L, et al. Changes in gut microbiota by chronic stress impair the efficacy of fluoxetine. Cell Rep. 2020;30(11):3682-3690.e6.

Metabolite

Glycine;

L-Isoleucine;

Serotonin;