Study M696
Study name | Yang JN 2021 |
Title | A green and efficient pseudotargeted lipidomics method for the study of depression based on ultra-high performance supercritical fluid chromatography-tandem mass spectrometry |
Overall design | In this study, a green andefficient pseudotargeted lipidomics method based on ultra-high performancesupercritical fluid chromatography-tandem mass spectrometry (UHPSFC-MS/MS) was developed. The UHPSFC-MS/MS-based pseudotargeted method was applied to the lipidomics study of the therapeutic effects of liquiritin on chronic unpredictable mild stress (CUMS) model of depression. Sprague-Dawley rats were divided into the following 4 groups: (1) control group, (2) CUMS group, (3) CUMS + liquiritin group (stressor plus liquiritin suspension treatment at the dose of 20 mg/kg), and (4) CUMS + fluoxetine group (stressor plus fluoxetine treatment at the dose of 10 mg/kg). The CUMS stress procedure lasted for 4 weeks, and drugs were administered via intragastric once per day during the model building period. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Not reported |
Sample size | 36 |
Tissue | Peripheral; Blood; Plasma; |
Platform | MS-based; LC-MS: LCMS-8060 mass spectrometer (Shimadzu, Japan); |
PMID | |
DOI | |
Citation | Yang J, Liu D, Jin W, et al. A green and efficient pseudotargeted lipidomics method for the study of depression based on ultra-high performance supercritical fluid chromatography-tandem mass spectrometry. J Pharm Biomed Anal. 2021;192:113646. |
Metabolite | Arachidonic acid; Docosahexaenoic acid; PE(18:0/18:2); Cer(d18:1/16:0); |
