Study M683
Study name | Zhang HC 2020 |
Title | An integrative metabolomics and network pharmacology method for exploring the effect and mechanism of Radix Bupleuri and Radix Paeoniae Alba on anti-depression |
Overall design | In this study, metabolomics and network pharmacology methods were used to explore the potential mechanism of antidepressant-like effects of the Radix Bupleuri and Radix Paeoniae Alba drug pair. Chronic unpredictable mild stress (CUMS) model of depression was established. Sprague-Dawley rats were divided into the following 6 groups (n = 10 in each group): (1) control group, (2) CUMS group, (3) CUMS + Xiaoyao Pill group (stressor plus Xiaoyao Pill treatment at the dose of 1.08 g/kg), (4) CUMS + low dose of Radix Bupleuri and Radix Paeoniae Alba drug pair group (stressor plus Radix Bupleuri and Radix Paeoniae Alba drug pair treatment at the dose of 1.0044 g/kg), (5) CUMS + middle dose of Radix Bupleuri and Radix Paeoniae Alba drug pair group (stressor plus Radix Bupleuri and Radix Paeoniae Alba drug pair treatment at the dose of 2.0088 g/kg), and (6) CUMS + high dose of Radix Bupleuri and Radix Paeoniae Alba drug pair group (stressor plus Radix Bupleuri and Radix Paeoniae Alba drug pair treatment at the dose of 4.0176 g/kg). The CUMS stress procedure lasted for 4 weeks, and drugs were administered via intragastric once per day during the model building period. Urine samples were used for metabonomic analysis. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test, forced swimming test |
Sample size | 60 |
Tissue | Peripheral; Urine; Urine; |
Platform | MS-based; LC-MS: AB-5600 HPLC-Q/TOF-MS (AB SCIEX); |
PMID | |
DOI | |
Citation | Zhang H, Zhang S, Hu M, et al. An integrative metabolomics and network pharmacology method for exploring the effect and mechanism of Radix Bupleuri and Radix Paeoniae Alba on anti-depression. J Pharm Biomed Anal. 2020;189:113435. |
Metabolite | Pyroglutamic acid; D-Glucose; Cyclic AMP; 3-Hydroxyanthranilic acid; 4-Trimethylammoniobutanoic acid; Cortisol; D-Xylose; Asymmetric dimethylarginine; Cortexolone; Deoxycorticosterone; Oleoyl-CoA; 10-Hydroxy-3-methoxy-1,3,5,7-cadinatetraen-9-one; 11-Dehydrocorticosterone; Pirbuterol; N-Acetyl-7-O-acetylneuraminic acid; Zalcitabine; N-Acetylhistidine; N6-Acetyl-L-lysine; Hydralazine; Atorvastatin; Oxytetracycline; Quercetin 4',7-diglucoside; Carbinoxamine; S-(1,2-Dichlorovinyl)glutathione; Norlaudanosoline; Terbutaline; Isoelemicin; Petasitenine; Oxamniquine; Falcarindiol 3-acetate; N-Acetylgalactosamine 4-sulphate; Formiminoglutamic acid; Hypericin; 1,2-Dichloroethane; |
