Study M681
Study name | Zhang HY 2020 |
Title | Serum metabolomics reveals the intervention mechanism and compatible regularity of Chaihu Shu Gan San on chronic unpredictable mild stress-induced depression rat model |
Overall design | The aim of this study was to provide a comprehensive study of the intervention mechanism and compatible regularity of Chaihu Shu Gan San (CSGS) and four compatibility groups (NJ, NC, NZ and NS) in a chronic unpredictable mild stress (CUMS)-induced depression model. Sprague-Dawley rats were divided into the following 7 groups (n = 6 in each group): (1) control group, (2) CUMS group, (3) CUMS + Chaihu Shu Gan San group (stressor plus Chaihu Shu Gan San treatment at the dose of 2.5 g/kg), (4) CUMS + NJ group (stressor plus NJ treatment at the dose of 2.5 g/kg), (5) CUMS + NC group (stressor plus NC treatment at the dose of 2.5 g/kg), (6) CUMS + NZ group (stressor plus NZ treatment at the dose of 2.5 g/kg), and (7) CUMS + NS group (stressor plus NS treatment at the dose of 2.5 g/kg). Drugs were administered via intragastric once per day for 40 days. After eight days of administration, rats in all groups except the control group were exposed to CUMS for 32 days. The metabolic profiles of serum samples were characterized by UPLC-Q-TOF/MS. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test |
Sample size | 42 |
Tissue | Peripheral; Blood; Serum; |
Platform | MS-based; LC-MS: Waters Acquity UPLC I-Class system (Waters Corp., Milford, MA, USA) with Waters definition accurate mass quadrupole time-of-flight (Q-TOF) Xevo G2-S mass spectrometer (Waters Corp., Milford, MA, USA); |
PMID | |
DOI | |
Citation | Zhang H, Huang H, Song H, et al. Serum metabolomics reveals the intervention mechanism and compatible regularity of Chaihu Shu Gan San on chronic unpredictable mild stress-induced depression rat model. J Pharm Pharmacol. 2020;72(8):1133-1143. |
Metabolite | Palmitic acid; Arachidonic acid; LysoPC(16:0); Phytosphingosine; LysoPC(18:0); 8,9-Epoxyeicosatrienoic acid; LysoPC(15:0/0:0); LysoPC(18:2(9Z,12Z)); Sphinganine; LysoPC(20:5(5Z,8Z,11Z,14Z,17Z)); Se-Adenosylselenohomocysteine; Triethanolamine; MG(0:0/16:1(9Z)/0:0); |
