Study M676
Study name | Yu M 2020a |
Title | Gut microbiota is the key to the antidepressant effect of Chaihu-Shu-Gan-San |
Overall design | The aim of this study was to investigate the antidepressant effects of Chaihu-Shu-Gan-San against chronic variable stress (CVS)-induced depression rats with and without antibiotic treatment using 16S rRNA gene sequencing and ultra-performance liquid chromatography coupled with time of flight mass spectrometry (UPLC-Q-TOF/MS) based metabolome approaches. Wistar rats were divided into the following 4 groups (n = 8 in each group): (1) control group, (2) CVS group, (3) CVS + antibiotic group (stressor plus oral administration dosed with antibiotic imipenem/cilastatin sodium at a daily dose of 75 mg/kg of body weight from day 25 to day 28), and (4) CVS + Chaihu-Shu-Gan-San group (stressor plus Chaihu-Shu-Gan-San water extracts by oral administration at the dose of 7.0 g/kg once daily for 28 days). The CVS stress procedure lasted for 4 weeks, and drugs were administered via intragastric once per day during the model building period. Hippocampal samples were used for metabonomic analysis. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Wistar rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test |
Sample size | 32 |
Tissue | Central; Brain; Hippocampus; |
Platform | MS-based; LC-MS: Waters Acquity Ultra Performance LC system (Waters Corp., Milford, MA, USA) with Waters SYNAPT G2 HDMS (Waters Corp., Manchester, UK) Q-TOF mass spectrometer; |
PMID | |
DOI | |
Citation | Yu M, Jia HM, Zhang T, et al. Gut microbiota is the key to the antidepressant effect of Chaihu-Shu-Gan-San. Metabolites. 2020;10(2):63. |
Metabolite | Gamma-Aminobutyric acid; Myristic acid; L-Phenylalanine; Glycine; L-Lactic acid; LysoPC(16:0); |
