Study M674
Study name | Yang JN 2020 |
Title | Enhanced pseudotargeted analysis using a segment data dependent acquisition strategy by liquid chromatography-tandem mass spectrometry for a metabolomics study of liquiritin in the treatment of depression |
Overall design | In this study, an enhanced pseudotargeted method using a segment data-dependent acquisition mode based on ultra-high performance liquid chromatography-tandem mass spectrometry was developed. The segment data dependent acquisition-based pseudotargeted method was successfully applied to the urine metabolomics study of the chronic unpredictable mild stress (CUMS) model of depression with the treatment of liquiritin. Sprague-Dawley rats were divided into the following 4 groups (n = 8 in each group): (1) control group, (2) CUMS group, (3) CUMS + liquiritin group (stressor plus liquiritin suspension treatment at the dose of 20 mg/kg), and (4) CUMS + fluoxetine group (stressor plus fluoxetine treatment at the dose of 10 mg/kg). The CUMS stress procedure lasted for 4 weeks, and drugs were administered via intragastric once per day during the model building period. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test, forced swimming test |
Sample size | 32 |
Tissue | Peripheral; Urine; Urine; |
Platform | MS-based; LC-MS: ACQUITY UHPLC I-Class system (Waters, Milford, MA, USA) and SYNAPT-G2S Q-TOF mass spectrometer (Waters, Milford, MA, USA); UHPLC-triple quadrupole MS (UHPLC-TQ MS) equipped with LCMS-8045 mass spectrometer (Shimadzu, Japan); |
PMID | |
DOI | |
Citation | Yang J, Jin W, Liu D, et al. Enhanced pseudotargeted analysis using a segment data dependent acquisition strategy by liquid chromatography-tandem mass spectrometry for a metabolomics study of liquiritin in the treatment of depression. J Sep Sci. 2020;43(11):2088-2096. |
Metabolite | L-Phenylalanine; Xanthurenic acid; N-Acetyl-L-glutamic acid; Aminoadipic acid; Melatonin; Glucosamine; Epinephrine; 10-Formyldihydrofolate; 1-Deoxynojirimycin; N(omega)-Hydroxyarginine; Mevalonic acid-5P; (Z)-4',6-Dihydroxyaurone; All-trans-13,14-dihydroretinol; (E,E)-Trichostachine; N1-(alpha-D-ribosyl)-5,6-dimethyl-benzimidazole; |
