Study M574
Study name | Yang R 2019 |
Title | Dietary of n-3 polyunsaturated fatty acids influence neurotransmitter systems of rats exposed to unpredictable chronic mild stress |
Overall design | The aim of this study was to investigate the effects of unpredictable chronic mild stress (CUMS) and n-3 polyunsaturated fatty acids (n-3 PUFAs) on central nervous system. High-performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) was used to quantify the levels of neurotransmitters and their metabolites involved in serotonergic, dopaminergic, noradrenergic, GABAergic neurotransmitter systems in hippocampus and prefrontal cortex. Sprague-Dawley rats were divided into the following 6 groups: (1) control group, (2) CUMS group, (3) control + n-3 PUFA deficiency group, (4) CMS + n-3 PUFA deficiency group, (5) control + n-3 PUFA supplementary group, and (6) CMS + n-3 PUFA supplementary group. The CUMS stress procedure lasted for 5 weeks. Six to seven samples per group were used for metabonomic analysis. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test, forced swimming test |
Sample size | 38 |
Tissue | Central; Brain; Hippocampus; Central; Brain; Prefrontal cortex; |
Platform | MS-based; LC-MS: Waters Acquity ultra performance liquid chromatography system (Waters, USA) with Micromass Quattro Premier XE tandem quadruple mass spectrometer (Waters, USA); |
PMID | |
DOI | |
Citation | Yang R, Zhang MQ, Xue Y, et al. Dietary of n-3 polyunsaturated fatty acids influence neurotransmitter systems of rats exposed to unpredictable chronic mild stress. Behav Brain Res. 2019;376:112172. |
Metabolite | Gamma-Aminobutyric acid; L-Glutamine; Homovanillic acid; 5-Hydroxyindoleacetic acid; |
