Study M558
Study name | Mou XJ 2019a |
Title | [Effects of Shugan Hewei Decoction and active substance fractions on behavior and neurotransmitter levels in hypothalamus of depression model rats] |
Overall design | The aim of this study was to investigate the effects of Shugan Hewei Decoction (SGHWT) and its active substance fractions (volatile oils, polysaccharides, and terpenoids) on behavior and neurotransmitter levels in hypothalamus of chronic unpredictable mild stress (CUMS) depression model rats. Sprague-Dawley rats were divided into the following 9 groups (n = 8 in each group): (1) control group, (2) CUMS group, (3) CUMS + fluoxetine group (stressor plus fluoxetine treatment at the dose of 1.58 g/kg), (4) CUMS + low dose of SGHWT group (stressor plus SGHWT treatment at the dose of 3.67 g/kg), (5) CUMS + high dose of SGHWT group (stressor plus SGHWT treatment at the dose of 14.68 g/kg), (6) CUMS + low dose of volatile oils group (stressor plus volatile oils treatment at the dose of 0.018 g/kg), (7) CUMS + high dose of volatile oils group (stressor plus volatile oils treatment at the dose of 0.073 g/kg), (8) CUMS + low dose of polysaccharides group (stressor plus polysaccharides treatment at the dose of 0.56 g/kg), and (9) CUMS + high dose of terpenoids group (stressor plus terpenoids treatment at the dose of 0.012 g/kg). The CUMS stress procedure lasted for 3 weeks, and drugs were administered via intragastric once per day for 1 week after stress. Several neurotransmitters in hypothalamus of rats were determined by LC-MS/MS method, including dopamine, norepinephrine, serotonin, 5-indoleacetic acid, GABA, and glutamic acid. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test, forced swimming test |
Sample size | 72 |
Tissue | Central; Brain; Hypothalamus; |
Platform | MS-based; LC-MS: LC-30AD liquid chromatography system (Shimadzu, Kyoto, Japan) coupled to 4500 AB Sciex QTRAP mass spectrometer (AB SCIEX, Foster City, CA, USA); |
PMID | |
DOI | |
Citation | Mou XJ, Liu H, Lin N, et al. [Effects of Shugan Hewei Decoction and active substance fractions on behavior and neurotransmitter levels in hypothalamus of depression model rats]. Zhongguo Zhong Yao Za Zhi. 2019;44(15):3343-8. |
Metabolite | Gamma-Aminobutyric acid; |
