Study M553
Study name | Liu XJ 2019 |
Title | Brain metabonomics study of the antidepressant-like effect of Xiaoyaosan on the CUMS-depression rats by 1 H NMR analysis |
Overall design | The aim of this study was to characterize the diversity and variation of two kinds metabolites of brain, i.e. aqueous and lipophilic metabolites, gaining comprehensive insights into the metabolic processes of depression-like behavior, and to reveal the mechanisms of antidepressant-like effects of Xiaoyaosan. A CUMS (chronic unpredictable mild stress)-induced depression-like behavior model was established. Both aqueous and lipophilic metabolites of rat brains was extracted by a two-phase extraction method, which were subsequently characterized by two differential sequences of 1H nuclear magnetic resonance. Sprague-Dawley rats were divided into the following 4 groups (n = 6 in each group): (1) control group, (2) CUMS group, (3) CUMS + Xiaoyaosan group (stressor plus Xiaoyaosan treatment at the dose of 46.2 g herb/kg), and (4) CUMS + venlafaxine group (stressor plus venlafaxine treatment at the dose of 25 mg/kg). The CUMS stress procedure lasted for 4 weeks, and drugs were administered via intragastric during the model building period. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test |
Sample size | 24 |
Tissue | Central; Brain; Brain; |
Platform | NMR; NMR: Bruker 600 MHz AVANC III spectrometer; |
PMID | |
DOI | |
Citation | Liu X, Zheng X, Du G, et al. Brain metabonomics study of the antidepressant-like effect of Xiaoyaosan on the CUMS-depression rats by 1 H NMR analysis. J Ethnopharmacol. 2019;235:141-154. |
Metabolite |
